阿尔茨海默病中的 microRNA：脑、脑脊液和血浆中的探索性研究。
MicroRNA in Alzheimer's disease: an exploratory study in brain, cerebrospinal fluid and plasma.
机构信息
Department of Geriatric (GRECC), Research, Education, and Clinical Centers, VA Puget Sound Health Care System, Seattle, WA 98108, USA.
出版信息
Biomarkers. 2013 Aug;18(5):455-66. doi: 10.3109/1354750X.2013.814073. Epub 2013 Jul 3.
Abstract
MicroRNA (miRNA) may be potential biomarkers of Alzheimer's disease (AD). The objective of this investigation was to demonstrate that miRNAs in human brain or biofluids are differentially expressed according to disease status, tissue type, neuritic plaque score or Braak stage. Post-mortem brain (PMB) miRNA were profiled using arrays and validated using quantitative RT-PCR (qRT-PCR). Five qRT-PCR-validated miRNAs were measured in an independent sample of PMB, cerebrospinal fluid and plasma from the same subjects. Plasma miR-15a was found to be associated with plaque score in the independent sample. In conclusion, miRNA present in human biofluids may offer utility as biomarkers of AD.
摘要
微 RNA（miRNA）可能是阿尔茨海默病（AD）的潜在生物标志物。本研究旨在证明人脑或生物体液中的 miRNA 根据疾病状态、组织类型、神经突斑块评分或 Braak 分期而存在差异表达。采用阵列技术对死后脑（PMB）miRNA 进行了分析，并采用定量 RT-PCR（qRT-PCR）进行了验证。在同一受试者的 PMB、脑脊液和血浆的独立样本中测量了 5 个 qRT-PCR 验证的 miRNA。在独立样本中发现血浆 miR-15a 与斑块评分相关。总之，存在于人体生物液中的 miRNA 可能可作为 AD 的生物标志物。
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2
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3
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