Barbul A, Lazarou S A, Efron D T, Wasserkrug H L, Efron G
Department of Surgery, Sinai Hospital, Baltimore, MD 21215.
Surgery. 1990 Aug;108(2):331-6; discussion 336-7.
Arginine has been shown to enhance wound healing and T-cell-mediated immune function in rodents. In this study the effect of oral arginine supplementation on human collagen synthesis and T-cell function was studied in 36 healthy, nonsmoking human volunteers. While volunteers were under local anesthesia, a 5 cm segment of expanded polytetrafluoroethylene tubing (1 mm outer diameter, 90 mu pore size) was inserted subcutaneously into the right deltoid region. The volunteers were then randomized into three groups that were given the following substances: (1) daily supplements of 30 gm arginine hydrochloride (24.8 gm free arginine); (2) 30 gm arginine aspartate (17 gm free arginine) daily; or (3) placebo. The supplements were given orally for 2 weeks; dietary intake was not controlled. Mitogenic responses of peripheral blood lymphocytes to phytohemagglutinin and concanavalin A were assayed at the start of study and at 1 and 2 weeks after supplementation. At 2 weeks the catheters were removed, and the amount of hydroxyproline was determined as an index of new collagen synthesis and deposition. Arginine supplementation significantly enhanced the amount of collagen deposited into a standardized wound as assessed by the amount of hydroxyproline present (10.1 +/- 2.32 nmol/cm graft in controls vs 17.57 +/- 2.16 nmol/cm in the arginine aspartate group, [p = 0.028] and vs 23.85 +/- 2.16 nmol/cm in the arginine hydrochloride group [p less than 0.001]). In parallel, arginine supplementation at both doses increased lymphocyte mitogenesis in response to phytohemagglutinin and concanavalin A. The data suggest that arginine may be of clinical benefit in improving wound healing and immune responses.
精氨酸已被证明可促进啮齿动物的伤口愈合和T细胞介导的免疫功能。在本研究中，对36名健康、不吸烟的人类志愿者进行了口服补充精氨酸对人体胶原蛋白合成和T细胞功能影响的研究。在志愿者处于局部麻醉状态下，将一段5厘米长的膨体聚四氟乙烯管（外径1毫米，孔径90微米）皮下插入右三角肌区域。然后将志愿者随机分为三组，分别给予以下物质：（1）每日补充30克盐酸精氨酸（游离精氨酸24.8克）；（2）每日补充30克天冬氨酸精氨酸（游离精氨酸17克）；或（3）安慰剂。补充剂口服2周；未控制饮食摄入。在研究开始时以及补充后1周和2周，测定外周血淋巴细胞对植物血凝素和刀豆球蛋白A的促有丝分裂反应。在2周时取出导管，并测定羟脯氨酸的量，作为新胶原蛋白合成和沉积的指标。通过存在的羟脯氨酸量评估，补充精氨酸显著增加了沉积到标准化伤口中的胶原蛋白量（对照组为10.1±2.32纳摩尔/厘米移植物，天冬氨酸精氨酸组为17.57±2.16纳摩尔/厘米，[p = 0.028]，盐酸精氨酸组为23.85±2.16纳摩尔/厘米，[p＜0.001]）。同时，两种剂量的精氨酸补充均增加了淋巴细胞对植物血凝素和刀豆球蛋白A的有丝分裂反应。数据表明，精氨酸在改善伤口愈合和免疫反应方面可能具有临床益处。
