Ho Emily L, Ronquillo Rollie, Altmeppen Hermann, Spudich Serena S, Price Richard W, Sinclair Elizabeth
Department of Neurology, Harborview Medical Center, University of Washington, Seattle, Washington, United States of America.
PLoS One. 2013 Jun 18;8(6):e66188. doi: 10.1371/journal.pone.0066188. Print 2013.
In order to characterize the cellular composition of cerebrospinal fluid (CSF) in a healthy state and in the setting of chronic pleocytosis associated with HIV-1 (HIV) infection, multi-parameter flow cytometry was used to identify and quantitate cellular phenotypes in CSF derived from HIV-uninfected healthy controls and HIV-infected subjects across a spectrum of disease and treatment. CD4+ T cells were the most frequent CSF population and the CD4:CD8 ratio was significantly increased in the CSF compared to blood (p = 0.0232), suggesting preferential trafficking of CD4+ over CD8+ T cells to this compartment. In contrast, in HIV-infection, CD8+ T cells were the major cellular component of the CSF and were markedly increased compared to HIV-uninfected subjects (p<0.001). As with peripheral blood, the CSF CD4:CD8 ratio was reversed in HIV-infected subjects compared to HIV-uninfected subjects. Monocytes, B cells and NK cells were rare in the CSF in both groups, although absolute counts of CSF NK cells and B cells were significantly increased in HIV-infected subjects (p<0.05). Our studies show that T cells are the major cellular component of the CSF in HIV-infected and uninfected subjects. The CSF pleocytosis characteristic of HIV infection involves all lymphocyte subsets we measured, except for CD4+ T cells, but is comprised primarily of CD8+ T cells. The reduced proportion of CD4+ T cells in the CSF may reflect both HIV-related peripheral loss and changes in trafficking patterns in response to HIV infection in the central nervous system.
为了描述健康状态下以及与人类免疫缺陷病毒1型（HIV）感染相关的慢性脑脊液细胞增多情况下脑脊液（CSF）的细胞组成，我们使用多参数流式细胞术来识别和定量来自未感染HIV的健康对照以及处于不同疾病阶段和治疗阶段的HIV感染受试者的脑脊液中的细胞表型。CD4⁺ T细胞是脑脊液中最常见的细胞群体，与血液相比，脑脊液中的CD4:CD8比值显著升高（p = 0.0232），这表明CD4⁺ T细胞比CD8⁺ T细胞更易进入该区域。相反，在HIV感染中，CD8⁺ T细胞是脑脊液的主要细胞成分，与未感染HIV的受试者相比显著增加（p<0.001）。与外周血一样，HIV感染受试者脑脊液中的CD4:CD8比值与未感染HIV的受试者相反。两组脑脊液中的单核细胞、B细胞和NK细胞均很少见，尽管HIV感染受试者脑脊液中NK细胞和B细胞的绝对计数显著增加（p<0.05）。我们的研究表明，T细胞是HIV感染和未感染受试者脑脊液中的主要细胞成分。HIV感染特有的脑脊液细胞增多涉及我们测量的所有淋巴细胞亚群，但不包括CD4⁺ T细胞，且主要由CD8⁺ T细胞组成。脑脊液中CD4⁺ T细胞比例的降低可能反映了与HIV相关的外周血损失以及中枢神经系统对HIV感染的反应中细胞转运模式的变化。
