Departments of Microbiology and Immunologya and Center for Biodefense and Emerging Infectious Disease,c University of Texas Medical Branch, Galveston, Texas, USA.
J Virol. 2013 Sep;87(17):9953-8. doi: 10.1128/JVI.01562-13. Epub 2013 Jul 3.
The newly emerged Middle East respiratory syndrome coronavirus (MERS-CoV) infects human bronchial epithelial Calu-3 cells. Unlike severe acute respiratory syndrome (SARS)-CoV, which exclusively infects and releases through the apical route, this virus can do so through either side of polarized Calu-3 cells. Infection results in profound apoptosis within 24 h irrespective of its production of titers that are lower than those of SARS-CoV. Together, our results provide new insights into the dissemination and pathogenesis of MERS-CoV and may indicate that the virus differs markedly from SARS-CoV.
新型中东呼吸综合征冠状病毒（MERS-CoV）感染人支气管上皮细胞 Calu-3。与严重急性呼吸综合征冠状病毒（SARS-CoV）不同，后者只能通过顶端途径感染和释放，这种病毒可以通过极化 Calu-3 细胞的任何一侧进行感染和释放。感染会导致细胞在 24 小时内发生明显的凋亡，而与 SARS-CoV 相比，其产生的病毒滴度较低。综上所述，我们的研究结果为 MERS-CoV 的传播和发病机制提供了新的见解，并可能表明该病毒与 SARS-CoV 有明显的不同。
