流体动力学尾静脉注射法作为研究肝脏启动子和增强子的一种工具。
The hydrodynamic tail vein assay as a tool for the study of liver promoters and enhancers.
作者信息
Kim Mee J, Ahituv Nadav
机构信息
Department of Bioengineering and Therapeutic Sciences, Institute for Human Genetics, University of California, San Francisco, CA, USA.
出版信息
Methods Mol Biol. 2013;1015:279-89. doi: 10.1007/978-1-62703-435-7_18.
Abstract
The hydrodynamic tail vein injection is a technique that is used to deliver nucleic acids into live mice. Delivery through this method results in the in vivo transfection of foreign DNA primarily in the liver. Here, we describe the use of this technique to test for regulatory activity of liver promoters and enhancers, using a dual luciferase reporter system as the readable/measureable output and how this application can be used for pharmacogenomic studies.
摘要
流体动力学尾静脉注射是一种用于将核酸导入活小鼠体内的技术。通过这种方法进行递送可使外源DNA主要在肝脏中进行体内转染。在此，我们描述了使用该技术，以双荧光素酶报告系统作为可读/可测量的输出，来检测肝脏启动子和增强子的调控活性，以及该应用如何用于药物基因组学研究。
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