de Oca Marcela Montes, Engwerda Christian, Haque Ashraful
Immunology and Infection Laboratory, Queensland Institute of Medical Research, Herston, QLD, Australia.
Methods Mol Biol. 2013;1031:203-13. doi: 10.1007/978-1-62703-481-4_23.
The term "severe malaria" refers to a wide spectrum of syndromes in Plasmodium-infected humans including cerebral malaria (CM), respiratory distress, severe anemia, liver dysfunction, and hypoglycemia. Mouse models have been employed to further our understanding of the pathology and immune responses that occur during Plasmodium infection. Evidence of brain, liver, lung, and spleen pathology, as well as anemia and tissue-sequestration of parasites, has been reported in various strains of inbred mice. While no single mouse model mimics all the various clinical manifestations of severe malaria in humans, here we describe a detailed protocol for Plasmodium berghei ANKA infection of C57BL/6J mice. For many years, this model has been referred to as "experimental cerebral malaria," but in fact recapitulates many of the symptoms and pathologies observed in most severe malaria syndromes.
“重症疟疾”一词指的是疟原虫感染人类后出现的一系列综合征，包括脑型疟疾（CM）、呼吸窘迫、严重贫血、肝功能障碍和低血糖。小鼠模型已被用于增进我们对疟原虫感染期间发生的病理和免疫反应的理解。在各种近交系小鼠品系中，已报告了脑、肝、肺和脾的病理证据，以及贫血和寄生虫的组织滞留情况。虽然没有单一的小鼠模型能模拟人类重症疟疾的所有临床表现，但在此我们描述了一种详细的伯氏疟原虫ANKA株感染C57BL/6J小鼠的方案。多年来，这个模型一直被称为“实验性脑型疟疾”，但实际上它概括了在大多数重症疟疾综合征中观察到的许多症状和病理情况。
