TNO Microbiology & Systems Biology, Zeist, The Netherlands; Netherlands Metabolomics Centre (NMC), Leiden, The Netherlands.
FEMS Yeast Res. 2013 Nov;13(7):609-17. doi: 10.1111/1567-1364.12062. Epub 2013 Aug 12.
To efficiently use lignocellulosic biomass hydrolysates as fermentation media for bioethanol production, besides being capable of producing significant amount of ethanol, the fermenting host should also meet the following two requirements: (1) resistant to the inhibitory compounds formed during biomass pretreatment process, (2) capable of utilizing C5 sugars, such as xylose, as carbon source. In our laboratory, a screening was conducted on microorganisms collected from environmental sources for their tolerance to hydrolysate inhibitors. A unique resistant strain was selected and identified as Pichia anomala (Wickerhamomyces anomalus), deposited as CBS 132101. The strain is able to produce ethanol in various biomass hydrolysates, both with and without oxygen. Besides, the strain could assimilate xylose and use nitrate as N source. These physiological characteristics make P. anomala an interesting strain for bioethanol production from lignocellulosic biomass hydrolysates.
为了有效地利用木质纤维素生物质水解物作为生物乙醇生产的发酵培养基，除了能够生产大量的乙醇外，发酵宿主还应满足以下两个要求：（1）能够抵抗生物质预处理过程中形成的抑制性化合物，（2）能够利用 C5 糖，如木糖，作为碳源。在我们的实验室中，对从环境来源收集的微生物进行了筛选，以测试它们对水解物抑制剂的耐受性。选择并鉴定了一株独特的抗性菌株，鉴定为异常毕赤酵母（Wickerhamomyces anomalus），保藏号为 CBS 132101。该菌株能够在各种生物质水解物中产生乙醇，无论是否有氧。此外，该菌株能够同化木糖并利用硝酸盐作为氮源。这些生理特性使异常毕赤酵母成为从木质纤维素生物质水解物生产生物乙醇的有趣菌株。
