MacLeod F A, Guiot S R, Costerton J W
Biotechnology Research Institute, National Research Council of Canada, Montreal, Quebec.
Appl Environ Microbiol. 1990 Jun;56(6):1598-607. doi: 10.1128/aem.56.6.1598-1607.1990.
The ultrastructure of bacterial granules that were maintained in an upflow anaerobic sludge bed and filter reactor was examined. The reactor was fed a sucrose medium, and it was operated at 35 degrees C. Scanning and transmission electron microscopy revealed that the granular aggregates were three-layered structures. The exterior layer of the granule contained a very heterogeneous population that included rods, cocci, and filaments of various sizes. The middle layer consisted of a slightly less heterogeneous population than the exterior layer. A more ordered arrangement, made up predominantly of bacterial rods, was evident in this second layer. The third layer formed the internal core of the granules. It consisted of large numbers of Methanothrix-like cells. Large cavities, indicative of vigorous gas production, were evident in the third layer. On the basis of these ultrastructural results, a model that presents a possible explanation of granule development is offered.
对在上流式厌氧污泥床和过滤反应器中维持的细菌颗粒的超微结构进行了检查。该反应器用蔗糖培养基进料，并在35℃下运行。扫描电子显微镜和透射电子显微镜显示，颗粒聚集体是三层结构。颗粒的外层包含非常异质的群体，包括各种大小的杆菌、球菌和丝状菌。中间层的群体异质性略低于外层。在这第二层中，明显存在一种更有序的排列，主要由杆菌组成。第三层形成颗粒的内部核心。它由大量类似甲烷丝状菌的细胞组成。在第三层中明显存在表明产气旺盛的大腔。基于这些超微结构结果，提供了一个对颗粒发育提出可能解释的模型。
