Dipartimento di Chimica, Sapienza Università di Roma, p.le Aldo Moro 5, 00185, Rome, Italy.
Analyst. 2013 Sep 7;138(17):5019-24. doi: 10.1039/c3an00806a. Epub 2013 Jul 5.
In this work we show the functionalization of the interior of microfluidic glass chips with poly(2-hydroxyethyl methacrylate) polymer brushes as anchors for co-immobilization of the enzymes glucose-oxidase and horseradish peroxidase. The formation of the brush layer and subsequent immobilization of these enzymes have been characterized on flat surfaces by atomic force microscopy and Fourier transform infrared spectroscopy, and studied inside glass chips by field emission scanning microscopy. Enzyme-functionalized glass chips have been applied for performing a multi-enzymatic cascade reaction for the fast (20 s) determination of glucose in human blood samples and the result is in excellent agreement with values obtained from the conventional hospital laboratory. The limit of detection of this bi-enzymatic method is 60 μM. With the advantages of high selectivity and reproducibility, this functionalization method can be used for improving the efficiency of glucose sensors.
在这项工作中，我们展示了将聚（2-羟乙基甲基丙烯酸酯）聚合物刷官能化到微流控玻璃芯片内部，作为共固定葡萄糖氧化酶和辣根过氧化物酶的锚点。通过原子力显微镜和傅里叶变换红外光谱在平面上对刷层的形成和这些酶的随后固定进行了表征，并通过场发射扫描显微镜在玻璃芯片内部进行了研究。酶功能化的玻璃芯片已用于进行多酶级联反应，以快速（20 秒）测定人血样中的葡萄糖，结果与传统医院实验室获得的值非常吻合。这种双酶法的检测限为 60 μM。这种功能化方法具有高选择性和重现性的优点，可用于提高葡萄糖传感器的效率。
