State Key Laboratory of Bioelectronic, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, People's Republic of China.
State Key Laboratory of Bioelectronic, School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, People's Republic of China.
Spectrochim Acta A Mol Biomol Spectrosc. 2014 May 5;125:183-8. doi: 10.1016/j.saa.2014.01.004. Epub 2014 Jan 18.
A novel glucose sensing strategy by using bi-enzyme coated monodispered silica nanoparticles (SiO2) was proposed. The monodispered SiO2 was synthesized according to our previously reported seed-growth methods. Glucose oxidase (GOD) and horseradish peroxidase (HRP) were simultaneously covalent immobilized on the surface of SiO2 nanoparticles through the cross-linker of glutaraldehyde. The immobilized bi-enzyme remained their bioactivities well for the substrate reaction. Thus, the resultant SiO2-GOD/HRP nanocomposites could be used as catalyst for enzymatic substrate reactions in the presence of 3,3',5,5'-tetramethylbenzidine (TMB) as chromogenic reagent and glucose as substrate. The factors of affecting the catalytic activities of enzymes were optimized. Under optimal conditions, the absorbance at 450 nm in UV-visible spectra increased with the glucose concentration, which could be used for glucose detection with a linear range from 0.5 μM to 250 μM and a detection limit of 0.22 μM at a signal-to-noise ratio of 3σ. Considering the potential of making pills using this SiO2-GOD/HRP, the present strategy has good prospect in the clinic science and other fields in future.
提出了一种新型的葡萄糖传感策略，该策略使用双酶涂层单分散二氧化硅纳米粒子 (SiO2)。单分散 SiO2 根据我们之前报道的种子生长方法合成。葡萄糖氧化酶 (GOD) 和辣根过氧化物酶 (HRP) 通过戊二醛交联剂同时共价固定在 SiO2 纳米粒子的表面。固定化双酶在基质反应中仍保持良好的生物活性。因此，所得的 SiO2-GOD/HRP 纳米复合材料可用作存在 3,3',5,5'-四甲基联苯胺 (TMB) 作为显色试剂和葡萄糖作为底物时酶基质反应的催化剂。优化了影响酶催化活性的因素。在最佳条件下，紫外可见光谱在 450nm 处的吸光度随着葡萄糖浓度的增加而增加，可用于葡萄糖检测，线性范围为 0.5μM 至 250μM，检测限为 0.22μM，信噪比为 3σ。考虑到使用这种 SiO2-GOD/HRP 制成药丸的潜力，该策略在未来的临床科学和其他领域具有良好的前景。
