Braun J, Schefels W, Stolla R
Gynäkologischen und Ambulatorischen Tierklinik der Universität München.
Berl Munch Tierarztl Wochenschr. 1990 Jun 1;103(6):211-2.
In 1970 semen from a Haflinger-stallion was frozen by the pellet method. 18 years later semen samples were used to inseminate 4 mares. Inseminations were performed shortly after ovulation with a total number of motile spermatozoa between 150 and 636 x 10(6), the percentage of motile spermatozoa being 20% to 40%. Three mares conceived after a single insemination, one mare got pregnant after 4 inseminations during 3 oestrous periods. Meanwhile, 3 foals were born and one of the mares is still pregnant. The results demonstrate that long-term storage of frozen semen in liquid nitrogen does not impair its fertilizing capacity.
1970年，一匹哈福林格种公马的精液采用颗粒法冷冻保存。18年后，精液样本用于给4匹母马输精。在排卵后不久进行输精，总活动精子数为150至636×10⁶，活动精子百分比为20%至40%。3匹母马单次输精后受孕，1匹母马在3个发情期进行4次输精后怀孕。同时，3匹小马驹出生，其中1匹母马仍怀有身孕。结果表明，液氮中长期保存冷冻精液不会损害其受精能力。
