Takeda Shiro, Kawahara Satoshi, Hidaka Muneaki, Yoshida Hiroki, Watanabe Wataru, Takeshita Masahiko, Kikuchi Yukiharu, Bumbein Dashnyam, Muguruma Michio, Kurokawa Masahiko
Research Division, Minami Nihon Rakuno Kyodo Co, Ltd, Moriya, Miyakonojo, Miyazaki 885-0003, Japan.
Biosci Biotechnol Biochem. 2013;77(7):1372-8. doi: 10.1271/bbb.120624. Epub 2013 Jul 7.
We investigated 10 lactic acid bacteria strains with probiotic potential prepared from Mongolian dairy products for their ability to induce T helper type-1 (Th1) cytokine production in mouse immune cells in vitro and in vivo. Among these strains, the Lactobacillus plantarum 06CC2 strain was effective in elevating the level of interleukin (IL)-12p40 in co-culture with J774.1 cells and the levels of IL-12 and interferon (IFN)-γ in co-culture with mouse spleen cells in vitro. Oral administration of this strain augmented the gene expression of IFN-γ and IL-12p40 and enlarged the population of CD4(+), CD25(+), and CD49b(+) cells in the spleens of normal mice. It also significantly elevated the gene expression of IL-12 receptor β2 as well as IL-12p40 and IFN-γ in Peyer's patches. Thus oral administration of strain 06CC2 was effective in inducing Th1 cytokine production activating the Th1 immune response associated with intestinal immunity in normal mice.
我们研究了从蒙古乳制品中制备的10株具有益生菌潜力的乳酸菌菌株，考察它们在体外和体内诱导小鼠免疫细胞产生1型辅助性T细胞（Th1）细胞因子的能力。在这些菌株中，植物乳杆菌06CC2菌株在与J774.1细胞共培养时能有效提高白细胞介素（IL）-12p40水平，在体外与小鼠脾细胞共培养时能提高IL-12和干扰素（IFN）-γ水平。口服该菌株可增强正常小鼠脾脏中IFN-γ和IL-12p40的基因表达，并增加CD4(+)、CD25(+)和CD49b(+)细胞的数量。它还显著提高了派尔集合淋巴结中IL-12受体β2以及IL-12p40和IFN-γ的基因表达。因此，口服06CC2菌株可有效诱导Th1细胞因子产生，激活正常小鼠与肠道免疫相关的Th1免疫反应。
