Vuorio A F, Sajantila A, Hämäläinen T, Syvänen A C, Ehnholm C, Peltonen L
Laboratory of Molecular Genetics, National Public Health Institute, Helsinki, Finland.
Biochem Biophys Res Commun. 1990 Jul 31;170(2):616-20. doi: 10.1016/0006-291x(90)92136-n.
Our purpose was to identify individuals from blood stains in two murder cases. We used primers flanking the hypervariable region of the apoB gene to amplify DNA extracted from blood stains and blood samples from suspected persons. The sensitivity and specificity of the procedure was improved by carrying out two consecutive PCR amplifications with a nested set of primers in the second amplification. The size of the generated fragments was determined by polyacrylamide gel electrophoresis followed by staining with ethidium bromide. By comparing the fragments produced from the stains with those from the blood samples we were able to identify the origin of the blood stains in both cases.
我们的目的是在两起谋杀案中从血迹中识别出个人。我们使用位于载脂蛋白B基因高变区两侧的引物，对从血迹和嫌疑人血样中提取的DNA进行扩增。通过在第二次扩增中使用一套嵌套引物进行连续两次聚合酶链反应（PCR）扩增，提高了该方法的灵敏度和特异性。通过聚丙烯酰胺凝胶电泳，然后用溴化乙锭染色，确定所产生片段的大小。通过将血迹产生的片段与血样产生的片段进行比较，我们能够在两起案件中确定血迹的来源。
