Department of Biomedical Sciences, Atlantic Veterinary College, Charlottetown, PE C1A 4P3, Canada.
Department of Chemistry, University of PEI, Charlottetown, PE C1A 4P3, Canada.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4294-4302. doi: 10.1099/ijs.0.051490-0. Epub 2013 Jul 5.
Two Gram-negative, facultatively anaerobic, rod-shaped bacteria, strains EF212(T) and PS125(T), were isolated from the octocorals Eunicea fusca and Plexaura sp., respectively. EF212(T) was isolated from a specimen of E. fusca collected off the coast of Florida, USA, and PS125(T) was isolated from a specimen of Plexaura sp. collected off the coast of Bimini, Bahamas. Analysis of the nearly full-length 16S rRNA gene sequences showed that these novel strains were most closely related to Endozoicomonas montiporae CL-33(T), E. elysicola MKT110(T) and E. numazuensis HC50(T) (EF212(T), 95.6-97.2 % identity; PS125(T), 95.1-96.4 % identity). DNA-DNA hybridization values among EF212(T), PS125(T), E. montiporae LMG 24815(T) and E. elysicola KCTC 12372(T) were far below the 70 % cut-off, with all values for duplicate measurements being less than 35 %. Both EF212(T) and PS125(T) required NaCl for growth and showed optimal growth at 2-3 % NaCl, 22-30 °C and pH 8.0. The predominant cellular fatty acids were summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c), summed feature 8 (C18 : 1ω6c and/or C18 : 1ω7c), C16 : 0 and C14 : 0. The DNA G+C content of EF212(T) was 48.6 mol% and that of PS125(T) was 47.5 mol%. In addition to the genotypic differences observed between the two novel strains and related type strains, phenotypic and chemotaxonomic experiments also revealed differences between strains. Thus, strains EF212(T) and PS125(T) represent novel species of the genus Endozoicomonas, for which the names Endozoicomonas euniceicola sp. nov. and Endozoicomonas gorgoniicola sp. nov., respectively, are proposed. The type strains are EF212(T) ( = NCCB 100458(T) = DSM 26535(T)) for Endozoicomonas euniceicola sp. nov. and PS125(T) ( = NCCB 100438(T) = CECT 8353(T)) for Endozoicomonas gorgoniicola sp. nov. An emended description of the genus Endozoicomonas is also provided to encompass differences observed in the results of genotypic, chemotaxonomic and phenotypic tests compared from the original and amended genus descriptions.
两株兼性厌氧、革兰氏阴性、杆状细菌，菌株 EF212(T) 和 PS125(T)，分别从软珊瑚 Eunicea fusca 和 Plexaura sp.中分离得到。EF212(T) 是从美国佛罗里达州海岸采集的 Eunicea fusca 标本中分离得到的，PS125(T) 是从巴哈马比米尼海岸采集的 Plexaura sp.标本中分离得到的。对近全长 16S rRNA 基因序列的分析表明，这些新菌株与内共生单胞菌 Endozoicomonas montiporae CL-33(T)、E. elysicola MKT110(T)和 E. numazuensis HC50(T)（EF212(T)，95.6-97.2%同源性；PS125(T)，95.1-96.4%同源性）最为密切相关。EF212(T)、PS125(T)、E. montiporae LMG 24815(T)和 E. elysicola KCTC 12372(T)之间的 DNA-DNA 杂交值远低于 70%的截止值，重复测量的所有值均小于 35%。EF212(T)和 PS125(T)都需要 NaCl 才能生长，在 2-3%NaCl、22-30°C 和 pH 8.0 时生长最佳。主要细胞脂肪酸为特征 3（C16:1ω6c 和/或 C16:1ω7c）、特征 8（C18:1ω6c 和/或 C18:1ω7c）、C16:0 和 C14:0。EF212(T)的 DNA G+C 含量为 48.6 mol%，PS125(T)的 DNA G+C 含量为 47.5 mol%。除了两株新菌株与相关模式菌株之间观察到的基因型差异外，表型和化学分类实验也揭示了菌株之间的差异。因此，菌株 EF212(T)和 PS125(T)代表内共生单胞菌属的新种，分别命名为内共生单胞菌 Euniceicola euniceicola sp. nov.和内共生单胞菌 Gorgoniicola gorgoniicola sp. nov.。模式菌株 EF212(T)（=NCCB 100458(T)=DSM 26535(T)）为 Euniceicola euniceicola sp. nov.，PS125(T)（=NCCB 100438(T)=CECT 8353(T)）为 Gorgoniicola gorgoniicola sp. nov.。还提供了对内共生单胞菌属的修订说明，以包含与原始和修订属描述相比，在基因型、化学分类和表型测试结果中观察到的差异。
