Institut für Angewandte Mikrobiologie, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Institute for Bioinformatics and Systems Biology, Justus-Liebig-Universität Giessen, D-35392 Giessen, Germany.
Syst Appl Microbiol. 2018 Mar;41(2):73-84. doi: 10.1016/j.syapm.2017.11.004. Epub 2017 Dec 22.
Two Gram-stain-negative, facultative anaerobic, motile, rod-shaped strains, S-B4-1U and JOB-63a, forming small whitish transparent colonies on marine agar, were isolated from a sponge of the genus Haliclona. The strains shared 99.7% 16S rRNA gene sequence identity and a DNA-DNA hybridization value of 100%, but were differentiated by genomic fingerprinting using rep-PCRs. 16S rRNA gene sequence phylogeny placed the strains as a sister branch to the monophyletic genus Endozoicomonas (Oceanospirillales; Gammaproteobacteria) with 92.3-94.3% 16S rRNA gene sequence similarity to Endozoicomonas spp., 91.9 and 92.1% to Candidatus Endonucleobacter bathymodiolin, and 91.9 to 92.1% to the type strains of Kistimonas spp. Core genome based phylogeny of strain S-B4-1U confirmed the phylogenetic placement. Major fatty acids were summed feature 3 (C ω7c/C ω6c) and 8 (C ω7c/C ω6c) followed by C 3-OH, C, and C. The G+C content was 50.1-51.4mol%. The peptidoglycan diamino acid of strain S-B4-1U was meso-diaminopimelic acid, the predominant polyamine spermidine, the major respiratory quinone ubiquinone Q-9; phosphatidylethanolamine, phosphatidylglycerol and phosphatidylserine were major polar lipids. Based on the clear phylogenetic distinction, the genus Parendozoicomonas gen. nov. is proposed, with Parendozoicomonas haliclonae sp. nov. as type species and strain S-B4-1U (=CCM 8713=DSM 103671=LMG 29769) as type strain and JOB-63a as a second strain of the species. Based on the 16S rRNA gene sequence phylogeny of the Oceanospirillales within the Gammaproteobacteria, the Endozoicomonaceae fam. nov. is proposed including the genera Endozoicomonas, Parendozoicomonas, and Kistimonas as well as the Candidatus genus Endonucleobacter.
两株革兰氏阴性、兼性厌氧、能动、杆状的菌株 S-B4-1U 和 JOB-63a 从海绵属 Haliclona 中分离得到，在海洋琼脂上形成小的白色透明菌落。这些菌株的 16S rRNA 基因序列相似度为 99.7%，DNA-DNA 杂交值为 100%，但通过重复序列 PCR 进行基因组指纹图谱分析则有所区别。16S rRNA 基因序列系统发育树将这些菌株置于单系属 Endozoicomonas（海洋螺旋菌目；γ-变形菌）的姐妹分支，与 Endozoicomonas spp. 的 16S rRNA 基因序列相似度为 92.3-94.3%，与 Candidatus Endonucleobacter bathymodiolin 的相似度为 91.9%和 92.1%，与 Kistimonas spp. 的模式株的相似度为 91.9-92.1%。菌株 S-B4-1U 的核心基因组系统发育树证实了其系统发育位置。主要脂肪酸为 summed feature 3 (C ω7c/C ω6c) 和 8 (C ω7c/C ω6c)，其次是 C 3-OH、C 和 C。G+C 含量为 50.1-51.4mol%。菌株 S-B4-1U 的肽聚糖二氨基庚二酸为 meso-二氨基庚二酸，主要多胺为亚精胺，主要呼吸醌为 ubiquinone Q-9；磷酯酰乙醇胺、磷酯甘油和磷酯丝氨酸为主要极性脂。基于清晰的系统发育差异，提出了 Parendozoicomonas 属，新种 Parendozoicomonas haliclonae sp. nov. 为模式种，菌株 S-B4-1U（=CCM 8713=DSM 103671=LMG 29769）为模式株，JOB-63a 为该种的第二株菌株。基于γ-变形菌中的海洋螺旋菌目 16S rRNA 基因序列系统发育树，提出了 Endozoicomonaceae 科，包括属 Endozoicomonas、Parendozoicomonas 和 Kistimonas 以及 Candidatus 属 Endonucleobacter。
