Laboratory of Microbiology, Department of Seafood Science, National Kaohsiung Marine University, 142 Hai-Chuan Road, Nan-Tzu, Kaohsiung City 811, Taiwan.
Yenepoya Research Center, Yenepoya University, Nithyananda Nagar, Deralakatee, Mangalore, Karnataka, India.
Int J Syst Evol Microbiol. 2010 May;60(Pt 5):1158-1162. doi: 10.1099/ijs.0.014357-0. Epub 2009 Aug 7.
A Gram-negative, aerobic, rod-shaped bacterium, designated strain CL-33(T), was isolated from the encrusting pore coral Montipora aequituberculata collected from seawater off the coast of southern Taiwan. 16S rRNA gene sequence analysis showed that the strain clustered closely with Endozoicomonas elysicola MKT110(T) (96.7 % similarity). The novel strain required NaCl for growth and exhibited optimal growth at 25 degrees C and in the presence of 2-3 % NaCl. Predominant cellular fatty acids were summed feature 3 (C(16 : 1)omega7c and/or C(16 : 1)omega6c; 39.6 %), summed feature 8 (C(18 : 1)omega7c and/or C(18 : 1)omega6c; 32.8 %) and C(16 : 0) (12.0 %). The DNA G+C content of strain CL-33(T) was 50.0 mol%. The results of physiological and biochemical tests allowed the clear phenotypic differentiation of this isolate from E. elysicola. It is evident from the genotypic, phenotypic and chemotaxonomic data presented that strain CL-33(T) represents a novel species of the genus Endozoicomonas, for which the name Endozoicomonas montiporae sp. nov. is proposed. The type strain is CL-33(T) (=LMG 24815(T) =BCRC 17933(T)).
一株革兰氏阴性、需氧、杆状细菌，被命名为 CL-33(T) 菌株，从台湾南部海域附着在多孔鹿角珊瑚上的硬珊瑚中分离得到。16S rRNA 基因序列分析表明，该菌株与内共生单胞菌属的 MKT110(T) 密切相关（相似度为 96.7%）。该新菌株的生长需要 NaCl，并在 25°C 和 2-3%NaCl 存在的条件下表现出最佳生长。主要细胞脂肪酸为求和特征 3（C(16 : 1)omega7c 和/或 C(16 : 1)omega6c；39.6%）、求和特征 8（C(18 : 1)omega7c 和/或 C(18 : 1)omega6c；32.8%）和 C(16 : 0)（12.0%）。CL-33(T) 菌株的 DNA G+C 含量为 50.0 mol%。生理生化测试的结果使该分离株与 E. elysicola 能够明显地在表型上进行区分。从呈现的基因型、表型和化学分类学数据可以明显看出，菌株 CL-33(T) 代表内共生单胞菌属的一个新种，现提议将其命名为内共生单胞菌 Montiporae sp. nov.。模式菌株为 CL-33(T)（=LMG 24815(T)=BCRC 17933(T)）。
