Institute of Soil Science and Photosynthesis RAS, Puschino, Moscow Region, Russia.
Curr Microbiol. 1993 Sep;27(3):181-4. doi: 10.1007/BF01576018.
The low-temperature, methane-oxidizing activities and species composition of methanotrophic communities in various tundra bog soils were investigated by radioisotopic and immunofluorescent methods. Methanotrophic bacteria carried out the methane oxidation process through all horizons of seasonally thawed layers down to permafrost. The highest activity of the process has been observed in the water surface layer of overmoistured soils and in water-logged moss covers. Up to 40% of(14)CH4 added was converted into(14)CO2, bacterial biomass, and organic exometabolites. By immunofluoresecent analysis it was demonstrated that the representatives of I+X (Methylomonas, Methylobacter, andMethylococcus) and II (Methylosinus, Methylocystis) methanotrophic groups occurred simultaneously in all samples at 61.6% and 38.4%, respectively. The number of methane-oxidizing bacteria in the ecosystems studied was 0.1-22.9×10(6) cells per gram of soil. Methanotrophic organisms ranged from 1% to 23% of the total bacterial number.
采用放射性同位素和免疫荧光方法研究了不同冻原沼泽土壤中低温、甲烷氧化活性和甲烷营养菌群落的物种组成。甲烷营养菌通过季节性解冻层的所有层次进行甲烷氧化过程，直至永冻层。在过度湿润土壤的水面层和水饱和苔藓覆盖层中观察到该过程的最高活性。添加的多达 40%的(14)CH4 被转化为(14)CO2、细菌生物量和有机外代谢物。通过免疫荧光分析表明，I+X（甲基单胞菌、甲基杆菌和甲基球菌）和 II（甲基杆菌、甲基囊泡菌）甲烷营养菌组的代表同时存在于所有样品中，分别为 61.6%和 38.4%。在所研究的生态系统中，甲烷氧化菌的数量为每克土壤 0.1-22.9×10(6)个细胞。甲烷营养菌占总细菌数量的 1%至 23%不等。
