Austin Suthanthiraraj Pearlson P, Graves Steven W
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque, New Mexico.
Curr Protoc Cytom. 2013 Jul;Chapter 1:1.2.1-1.2.14. doi: 10.1002/0471142956.cy0102s65.
The use of fluidics is implicit in a technology named "flow cytometry," which flows a cell or particle through a sensing volume to obtain serial analysis of particles on a one by one basis. This flow of particles enables flow cytometry to collect information on multiple particle populations, giving it a distinct advantage over bulk analysis approaches. Moreover, flow cytometers can analyze thousands of particles per second in a single flowing stream. Additionally, use of volumetric sample delivery makes it possible for flow cytometers to accurately count cells and particles. Furthermore, the analysis results can be coupled with a fluidic diversion mechanism to sort and collect particles based on desired properties. Finally, when high-throughput sampling technologies are employed to rapidly change the input of the sample stream, a flow cytometer can become an integral tool for high-throughput screening. The above properties have made flow cytometry useful in a wide range of biomedical applications. In this unit we will present an overview of fluidic systems that make flow cytometry possible. This will introduce historical approaches, explanations of the commonly implemented current fluidics, and brief discussions of potential future fluidics where appropriate.
在一种名为“流式细胞术”的技术中，流体ics的应用是隐含的，该技术使细胞或颗粒流过一个传感体积，以便逐个对颗粒进行连续分析。这种颗粒流使流式细胞术能够收集多个颗粒群体的信息，这使其相对于批量分析方法具有明显优势。此外，流式细胞仪每秒可在单个流动流中分析数千个颗粒。此外，使用体积式样品输送使流式细胞仪能够准确计数细胞和颗粒。此外，分析结果可与流体分流机制相结合，根据所需特性对颗粒进行分选和收集。最后，当采用高通量采样技术快速改变样品流的输入时，流式细胞仪可成为高通量筛选的重要工具。上述特性使流式细胞术在广泛的生物医学应用中发挥作用。在本单元中，我们将概述使流式细胞术成为可能的流体系统。这将介绍历史方法、对当前常用流体ics的解释，并在适当情况下简要讨论潜在的未来流体ics。
