使用微吸管技术对培养细胞（HeLa细胞和骨髓瘤细胞）的膜电容进行频域分析。
Frequency domain analysis of membrane capacitance of cultured cells (HeLa and myeloma) using the micropipette technique.
作者信息
Asami K, Takahashi Y, Takashima S
机构信息
Institute for Chemical Research, Kyoto University, Japan.
出版信息
Biophys J. 1990 Jul;58(1):143-8. doi: 10.1016/S0006-3495(90)82360-2.
Abstract
The membrane capacitance and conductance of cultured cells (HeLa and mouse myeloma) are investigated using the micropipette method. Mean values of the membrane capacities were found to be 1.9 microF/cm2 for HeLa cells and 1.0 microF/cm2 for myeloma cells. These values are in agreement with those obtained using the suspension method. Whereas the suspension method is unable to provide the information on membrane conductance, the micropipette method is able to measure even an extremely small membrane conductance if leakage current is negligibly small. The membrane conductances were found, using this technique, to be approximately 90-100 microS/cm2 for both HeLa and myeloma cells. One of the purposes of this study is to establish the frequency profile of membrane capacitance. It was found, however, that membrane capacitances of these cells are independent of frequency between 1 Hz and 1 KHz within the resolution of this technique.
摘要
使用微吸管法研究了培养细胞（HeLa细胞和小鼠骨髓瘤细胞）的膜电容和电导。发现HeLa细胞的膜电容平均值为1.9微法/平方厘米，骨髓瘤细胞为1.0微法/平方厘米。这些值与使用悬浮法获得的值一致。虽然悬浮法无法提供有关膜电导的信息，但如果漏电流小到可以忽略不计，微吸管法甚至能够测量极小的膜电导。使用该技术发现，HeLa细胞和骨髓瘤细胞的膜电导均约为90 - 100微西门子/平方厘米。本研究的目的之一是确定膜电容的频率分布。然而，发现在该技术的分辨率范围内，这些细胞的膜电容在1赫兹至1千赫兹之间与频率无关。
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