Asami K, Takahashi Y, Takashima S
Department of Bioengineering, University of Pennsylvania, Philadelphia.
Biochim Biophys Acta. 1989 Jan 17;1010(1):49-55. doi: 10.1016/0167-4889(89)90183-3.
In order to study the effect of the nucleus on dielectric behavior of the whole cell, permittivity (dielectric constant) and conductivity of mouse lymphocytes and erythrocytes were measured over a frequency range from 0.1 to 250 MHz. Erythrocytes (spherocytes) showed a single dielectric dispersion, which was explained by a single-shell model that is a conducting sphere covered with a thin insulating shell. On the other hand, lymphocytes showed a broad dielectric dispersion curve which was composed of two subdispersions. The high-frequency subdispersion, which was not found for erythrocytes, was assigned to the Maxwell-Wagner dispersion of the nucleus occupying about 65% of the total cell volume. Analysis of the lymphocyte dispersion was carried out by a double-shell model, in which a shelled sphere, i.e., nucleus, is incorporated into the single-shell model. The following electrical parameters were consequently estimated; the capacitance of the plasma membrane, 0.86 microF.cm-2; the conductivity of the cytoplasm, 3.2 mS.cm-1; the capacitance and conductance of the nuclear envelope are, respectively, 0.62 microF.cm-2 and 15 S.cm-2, and the permittivity and conductivity of the nucleoplasm are 52 and 13.5 mS.cm-1.
为了研究细胞核对整个细胞介电行为的影响，在0.1至250 MHz的频率范围内测量了小鼠淋巴细胞和红细胞的电容率（介电常数）和电导率。红细胞（球形红细胞）呈现出单一的介电色散，这可以用单壳模型来解释，即一个被薄绝缘壳覆盖的导电球体。另一方面，淋巴细胞呈现出一条由两个子色散组成的宽介电色散曲线。红细胞未出现的高频子色散被归因于占细胞总体积约65%的细胞核的麦克斯韦-瓦格纳色散。通过双壳模型对淋巴细胞色散进行分析，其中将一个带壳球体，即细胞核，纳入单壳模型。由此估计出以下电学参数：质膜电容为0.86微法·厘米⁻²；细胞质电导率为3.2毫西门子·厘米⁻¹；核膜的电容和电导分别为0.62微法·厘米⁻²和15西门子·厘米⁻²，核质的电容率和电导率分别为52和13.5毫西门子·厘米⁻¹。
