Abney J R, Scalettar B A, Hackenbrock C R
Department of Cell Biology and Anatomy, University of North Carolina, Chapel Hill 27599-7090.
Biophys J. 1990 Jul;58(1):261-5. doi: 10.1016/S0006-3495(90)82371-7.
Interparticle interactions are incorporated into the theoretical description of the initial amplitude, G(0), of the normalized fluorescence correlation spectroscopy autocorrelation function. Measurements of particle number, aggregate size, and interaction-dependent diffusion are then analyzed in the context of this generalized theory. It is shown that the neglect of interactions can introduce order-of-magnitude errors into estimates of particle number and aggregate size. It is also shown that measurement of G(0) provides an essentially unique method for testing the validity of theories of interaction-dependent membrane protein diffusion.
粒子间相互作用被纳入到归一化荧光相关光谱自相关函数初始振幅G(0)的理论描述中。然后，在这个广义理论的背景下分析粒子数、聚集体大小以及相互作用依赖扩散的测量结果。结果表明，忽略相互作用会在粒子数和聚集体大小的估计中引入数量级误差。还表明，对G(0)的测量提供了一种本质上独特的方法来检验相互作用依赖膜蛋白扩散理论的有效性。
