Boston, Mass. From the Division of Plastic Surgery and the Department of Pathology, Brigham and Women's Hospital, Harvard Medical School.
Plast Reconstr Surg. 2012 Mar;129(3):443e-452e. doi: 10.1097/PRS.0b013e318241289c.
Major loss of skin following burns or trauma requires skin grafting for repair. In addition, chronic wounds frequently require skin grafts. Current treatments are either cumbersome, limited in possible expansion ratio, costly, or require extensive time for treatment. This study investigates a new way of regenerating skin after major burns and other trauma, providing 100-fold expansion of a split-thickness skin graft.
Submillimeter micrografts were created by controlled mincing of a split-thickness skin graft and transplanted to porcine full-thickness wounds. By creating an incubator-like microenvironment using wound chambers, the micrografts provide reepithelialization whether placed dermal side up or dermal side down.
Transplantation of micrografts in a 1:100 expansion ratio results in complete epithelialization of both healthy and diabetic wounds within 14 days. In comparison, nontransplanted wounds showed 62 percent reepithelialization in healthy pigs and 49 percent in diabetic pigs at the corresponding time point.
Minced skin micrografts are very effective in wound repair and can provide 100-fold expansion of a skin graft. Early clinical results confirm the utility of this technique.
烧伤或创伤后大面积皮肤缺失需要进行皮肤移植修复。此外，慢性创面也常需要皮肤移植。目前的治疗方法要么繁琐，扩张比有限，要么昂贵，要么需要大量的治疗时间。本研究探讨了一种新的方法，即在大面积烧伤和其他创伤后再生皮肤，可使皮片面积扩大 100 倍。
通过控制将皮片切成微小的微皮片，并移植到猪的全层创面，从而创造出亚毫米级别的微皮片。通过使用创腔室创造出类似于孵育器的微环境，微皮片无论是皮面朝上还是皮面朝下放置，都能重新上皮化。
以 1:100 的比例移植微皮片，可在 14 天内使健康和糖尿病创面完全上皮化。相比之下，未移植的创面在相应的时间点，健康猪的上皮化率为 62%，糖尿病猪为 49%。
切碎的皮肤微皮片在伤口修复中非常有效，可使皮片面积扩大 100 倍。早期的临床结果证实了该技术的实用性。
