Fuchs Christiane, Pham Linh, Wang Ying, Farinelli William A, Anderson R Rox, Tam Joshua
Wellman Center for Photomedicine, Massachusetts General Hospital, Boston, MA 02114, USA.
Department of Dermatology, Harvard Medical School, Boston, MA 02115, USA.
Sci Adv. 2021 Oct 8;7(41):eabj0864. doi: 10.1126/sciadv.abj0864.
Skin wounds are immense medical and socioeconomic burdens, and autologous skin grafting remains the gold standard for wound repair. We recently found that full-thickness micro skin tissue columns (MSTCs) can be harvested with minimal donor site morbidity, and that MSTCs applied to wounds “randomly” (without maintaining their natural epidermal-dermal orientation) can accelerate re-epithelialization. However, despite MSTCs containing all the cellular and extracellular contents of full-thickness skin, normal dermal architecture was not restored by random MSTCs. In this study, we developed a magnetically induced assembly method to produce constructs of densely packed, oriented MSTCs that closely resemble the overall architecture of full-thickness skin to test the hypothesis that maintaining MSTCs’ orientation could further hasten healing and restore a normal dermis. Our method led to faster and more orderly re-epithelialization but unexpectedly did not improve the retention of dermal architecture, which reveals a hitherto unappreciated role for tissue morphology in determining dermal remodeling outcomes.
皮肤伤口带来了巨大的医学和社会经济负担，自体皮肤移植仍然是伤口修复的金标准。我们最近发现，可以以最小的供体部位发病率获取全层微型皮肤组织柱（MSTC），并且将MSTC“随机”（不保持其自然的表皮-真皮方向）应用于伤口可加速再上皮化。然而，尽管MSTC包含全层皮肤的所有细胞和细胞外成分，但随机植入的MSTC并未恢复正常的真皮结构。在本研究中，我们开发了一种磁诱导组装方法，以生产紧密堆积、定向的MSTC构建体，其与全层皮肤的整体结构非常相似，以检验维持MSTC方向可进一步加速愈合并恢复正常真皮的假设。我们的方法导致了更快、更有序的再上皮化，但出乎意料的是，并没有改善真皮结构的保留情况，这揭示了组织形态在决定真皮重塑结果中迄今未被认识到的作用。
