Central Veterinary Institute of Wageningen UR, P.O. Box 65, 8200 AB Lelystad, The Netherlands.
Vet Microbiol. 2013 Sep 27;166(1-2):195-9. doi: 10.1016/j.vetmic.2013.06.008. Epub 2013 Jun 21.
Bovine viral diarrhea virus (BVDV) belongs together with Classical swine fever virus (CSFV) and Border disease virus (BDV) to the genus Pestivirus in the Flaviviridae family. BVDV has been subdivided into two different species, BVDV1 and BVDV2 based on phylogenetic analysis. Subsequent characterization of both strains revealed major antigenic differences. Because the envelope glycoprotein E2 is the most immunodominant protein for all pestiviruses, the present study focused on epitope mapping by constructing chimeric BVDV type 1 and 2 E2 genes in expression plasmids. These plasmids with chimeric E2-genes were transfected in SK6 cells and transient expression was studied by immunostaining with a panel of MAbs specific for E2 of BVDV1 or BVDV2, resulting in the localization of type-specific antigenic domains at similar regions. These results indicate that E2 glycoproteins of both BVDV types exhibit a comparable antigenic structure, but with type specific epitopes. In addition, the antigenic resemblance with envelope glycoprotein E2 of Classical swine fever virus is discussed.
牛病毒性腹泻病毒（BVDV）与经典猪瘟病毒（CSFV）和边界病病毒（BDV）一起属于黄病毒科瘟病毒属。根据系统发育分析，BVDV 已分为两个不同的种，即 BVDV1 和 BVDV2。随后对这两种毒株的特征分析显示出主要的抗原差异。由于包膜糖蛋白 E2 是所有瘟病毒中最具免疫原性的蛋白，因此本研究通过在表达质粒中构建 BVDV 1 型和 2 型的嵌合 E2 基因，重点进行了表位作图。将这些带有嵌合 E2 基因的质粒转染到 SK6 细胞中，并通过用针对 BVDV1 或 BVDV2 E2 的 MAbs 进行免疫染色来研究瞬时表达，结果表明，两种 BVDV 类型的 E2 糖蛋白在相似区域具有特定于类型的抗原表位。这些结果表明，两种 BVDV 类型的 E2 糖蛋白均表现出可比较的抗原结构，但具有特定于类型的表位。此外，还讨论了与经典猪瘟病毒包膜糖蛋白 E2 的抗原相似性。
