Department of Virology, National Veterinary Research Institute, Al. Partyzantów 57, 24-100, Puławy, Poland.
Virus Genes. 2020 Aug;56(4):515-521. doi: 10.1007/s11262-020-01756-2. Epub 2020 Apr 16.
Bovine viral diarrhea virus (BVDV) belongs to the Pestivirus genus of the Flaviviridae family and has worldwide distribution, being one of the main causes of economic losses in cattle raising. The genome of pestiviruses is a single strand of positive-sense RNA with a length of 12.3 kb, which encodes one open reading frame flanked by untranslated regions. E2 glycoprotein is required for binding to cell-surface receptors and it also contains major antigenic determinants. The nucleotide sequence coding E2 is the most variable part of the viral genome. The heterogeneity that exists among circulating strains causes problems in the development of effective vaccines and reliable diagnostics. In this study, and for the first time analysis was made of the E2 glycoprotein coding sequences of 14 Polish BVDV-1 strains which belong to four subtypes: 1b (n = 7), 1f (n = 3), 1s (n = 3), and 1r (n = 1). These sequences showed evidence of strong purifying (negative) selection. However, we also identified positively selected sites. The availability of E2 sequences of Polish BVDV strains for reference, knowledge gained through epitope prediction attempts, and information on protein glycosylation sites can afford a better understanding of host-pathogen interactions.
牛病毒性腹泻病毒（BVDV）属于黄病毒科瘟病毒属，具有世界分布性，是导致养牛业经济损失的主要原因之一。瘟病毒的基因组是一条长度为 12.3kb 的单链正链 RNA，编码一个开放阅读框，两侧为非翻译区。E2 糖蛋白是结合细胞表面受体所必需的，它还包含主要的抗原决定簇。E2 编码的核苷酸序列是病毒基因组中最易变的部分。循环毒株之间的异质性导致有效疫苗和可靠诊断的开发出现问题。在这项研究中，首次对属于四个亚型的 14 株波兰 1 型 BVDV-1 株（1b[n=7]、1f[n=3]、1s[n=3]和 1r[n=1]）的 E2 糖蛋白编码序列进行了分析。这些序列显示出强烈的纯化（负）选择证据。然而，我们也确定了正选择位点。波兰 BVDV 株的 E2 序列可作为参考，通过尝试预测抗原表位获得的知识，以及关于蛋白质糖基化位点的信息，可以更好地了解宿主-病原体相互作用。
