Deregt D, van Rijn P A, Wiens T Y, van den Hurk J
Animal Diseases Research Institute, Canadian Food Inspection Agency, Lethbridge, Alberta.
Virus Res. 1998 Oct;57(2):171-81. doi: 10.1016/s0168-1702(98)00095-1.
Bovine viral diarrhea virus (BVDV) has recently been segregated into two genotypes, namely, BVDV 1 and BVDV 2. Viruses of the BVDV 2 genotype are a cause of hemorrhagic and acute fatal disease in cattle in the US and Canada. In this study, monoclonal antibodies (mAbs) to the newly described BVDV 2 were produced after immunization with virus or a combination of virus and E2 peptide. From an original panel of 17 mAbs, 13 mAbs were identified as E2-specific by reactivity with a BVDV 2 recombinant E2 protein expressed in insect cells. Nine E2 mAbs were observed to be virus-neutralizing. The E2 epitopes represented by the mAbs were found to be highly conserved among BVDV 2 isolates associated with hemorrhagic or severe disease in cattle. Except for one virus-neutralizing E2 mAb, the mAbs showed few or relatively weak cross-reactions with BVDV 1. Two non-neutralizing E2 mAbs were BVDV 2-specific. In contrast to BVDV 1 for which conserved neutralizing epitopes have been mapped in one immunodominant domain, the virus-neutralizing E2 mAbs produced to BVDV 2 were found to bind to highly conserved epitopes in three antigenic domains.
牛病毒性腹泻病毒（BVDV）最近被分为两种基因型，即BVDV 1和BVDV 2。BVDV 2基因型病毒是美国和加拿大牛群中出血性和急性致命疾病的病因。在本研究中，用病毒或病毒与E2肽的组合免疫后，产生了针对新描述的BVDV 2的单克隆抗体（mAb）。在最初的17种mAb中，通过与在昆虫细胞中表达的BVDV 2重组E2蛋白反应，鉴定出13种mAb为E2特异性。观察到9种E2 mAb具有病毒中和作用。发现mAb所代表的E2表位在与牛出血性或严重疾病相关的BVDV 2分离株中高度保守。除一种病毒中和E2 mAb外，这些mAb与BVDV 1的交叉反应很少或相对较弱。两种非中和E2 mAb是BVDV 2特异性的。与已在一个免疫显性结构域中定位了保守中和表位的BVDV 1不同，发现针对BVDV 2产生的病毒中和E2 mAb与三个抗原结构域中的高度保守表位结合。
