Kim Moon Jung, Bae Il Kwon, Jeong Seok Hoon, Kim So Hyun, Song Jae Hoon, Choi Jae Young, Yoon Sang Sun, Thamlikitkul Visanu, Hsueh Po-Ren, Yasin Rohani Md, Lalitha M K, Lee Kyungwon
Department of Biomedical Laboratory Science, Eulji University College of Health Science, Seongnam, Korea.
J Antimicrob Chemother. 2013 Dec;68(12):2820-4. doi: 10.1093/jac/dkt269. Epub 2013 Jul 9.
To investigate the epidemiological traits of metallo-β-lactamase (MBL)-producing Pseudomonas aeruginosa (MPPA) clinical isolates collected by the Asian Network for Surveillance of Resistant Pathogens (ANSORP).
A total of 16 MPPA clinical isolates were collected from six Asian countries in 2000 to 2009 by ANSORP. The MBL gene was detected by PCR amplification. The genetic organization of the class 1 integron carrying the MBL gene cassette was investigated by PCR mapping and sequencing. Southern blotting, repetitive sequence-based PCR and multilocus sequence typing (MLST) experiments were performed to characterize the isolates.
PCR and sequencing experiments detected the blaVIM-2 (n = 12), blaVIM-3 (n = 1), blaIMP-6 (n = 2) and blaIMP-26 (n = 1) genes. The MBL genes were located on the chromosome in all isolates except one. Furthermore, all the MBL genes were located in a class 1 integron. All the MPPA isolates from Malaysia, Thailand, Sri Lanka and Korea were identified as sequence type (ST) 235 by MLST. Three VIM-2-producing isolates from India were identified as ST773, and one isolate harbouring VIM-3 from Taiwan was identified as ST298.
P. aeruginosa ST235 might play a role in dissemination of MBL genes in Asian countries.
调查亚洲耐药病原体监测网络（ANSORP）收集的产金属β-内酰胺酶（MBL）的铜绿假单胞菌（MPPA）临床分离株的流行病学特征。
2000年至2009年期间，ANSORP从6个亚洲国家共收集了16株MPPA临床分离株。通过PCR扩增检测MBL基因。通过PCR图谱分析和测序研究携带MBL基因盒的1类整合子的遗传结构。进行Southern印迹、基于重复序列的PCR和多位点序列分型（MLST）实验以对分离株进行特征分析。
PCR和测序实验检测到blaVIM-2（n = 12）、blaVIM-3（n = 1）、blaIMP-6（n = 2）和blaIMP-26（n = 1）基因。除1株外，所有分离株中的MBL基因均位于染色体上。此外，所有MBL基因均位于1类整合子中。通过MLST分析，来自马来西亚、泰国、斯里兰卡和韩国的所有MPPA分离株均被鉴定为序列型（ST）235。来自印度的3株产VIM-2分离株被鉴定为ST773，来自台湾的1株携带VIM-3的分离株被鉴定为ST298。
铜绿假单胞菌ST235可能在亚洲国家MBL基因的传播中起作用。
