Cho Suhyung, Cho Yoobok, Lee Sooin, Kim Jayoung, Yum Hyeji, Kim Sun Chang, Cho Byung-Kwan
Department of Biological Sciences and KAIST Institute for the BioCentury, Intelligent Synthetic Biology Center, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea.
Genomics Inform. 2013 Jun;11(2):76-82. doi: 10.5808/GI.2013.11.2.76. Epub 2013 Jun 30.
Over the past decade or so, dramatic developments in our ability to experimentally determine the content and function of genomes have taken place. In particular, next-generation sequencing technologies are now inspiring a new understanding of bacterial transcriptomes on a global scale. In bacterial cells, whole-transcriptome studies have not received attention, owing to the general view that bacterial genomes are simple. However, several recent RNA sequencing results are revealing unexpected levels of complexity in bacterial transcriptomes, indicating that the transcribed regions of genomes are much larger and complex than previously anticipated. In particular, these data show a wide array of small RNAs, antisense RNAs, and alternative transcripts. Here, we review how current transcriptomics are now revolutionizing our understanding of the complexity and regulation of bacterial transcriptomes.
在过去十年左右的时间里，我们通过实验确定基因组内容和功能的能力取得了巨大进展。特别是，新一代测序技术正在促使人们在全球范围内对细菌转录组有新的认识。在细菌细胞中，全转录组研究一直未受到关注，因为人们普遍认为细菌基因组很简单。然而，最近的一些RNA测序结果揭示了细菌转录组中意想不到的复杂程度，表明基因组的转录区域比以前预期的要大得多且复杂得多。特别是，这些数据显示了各种各样的小RNA、反义RNA和可变转录本。在这里，我们回顾当前的转录组学如何正在彻底改变我们对细菌转录组复杂性和调控的理解。
