Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences - WULS, Nowoursynowska 159, 02-776, Warsaw, Poland.
BMC Vet Res. 2013 Jul 11;9:138. doi: 10.1186/1746-6148-9-138.
Spontaneous canine mammary tumors constitute a serious clinical problem. There are significant differences in survival between cases with different tumor grades. Unfortunately, the distinction between various grades is not clear. A major problem in evaluating canine mammary cancer is identifying those, that are "truly" malignant. That is why the aim of our study was to find the new markers of canine malignancy, which could help to diagnose the most malignant tumors.
Analysis of gene expression profiles of canine mammary carcinoma of various grade of malignancy followed by the boosted tree analysis distinguished a gene set. The expression of this gene set (sehrl, zfp37, mipep, relaxin, and magi3) differs significantly in the most malignant tumors at mRNA level as well as at protein level. Despite this gene set is very interesting as an additional tool to estimate canine mammary malignancy, it should be validated using higher number of samples.
The proposed gene set can constitute a malignancy marker that could help to distinguish the most malignant canine mammary carcinomas. These genes are also interesting as targets for further investigations and therapy. So far, only two of them were linked with the cancer development.
自发性犬乳腺肿瘤是一个严重的临床问题。不同肿瘤分级的病例之间的存活率有显著差异。不幸的是，不同分级之间的区别并不明显。评估犬乳腺肿瘤的一个主要问题是确定哪些是“真正”恶性的。这就是为什么我们的研究目的是寻找犬恶性肿瘤的新标志物，以帮助诊断最恶性的肿瘤。
对不同恶性程度的犬乳腺肿瘤的基因表达谱进行分析，然后进行提升树分析，区分出一个“基因集”。该基因集（sehrl、zfp37、mipep、松弛素和 magi3）在 mRNA 水平和蛋白质水平上在最恶性的肿瘤中的表达差异显著。尽管该“基因集”作为评估犬乳腺恶性程度的额外工具非常有趣，但应该使用更多的样本进行验证。
所提出的基因集可以作为一个“恶性标志物”，有助于区分最恶性的犬乳腺癌。这些基因也作为进一步研究和治疗的目标很有趣。到目前为止，只有其中两个与癌症的发展有关。
