溶质载体家族41成员1（SLC41A1）是唯一在子痫前期孕妇胎盘组织中显著高表达的镁反应基因。
SLC41A1 is the only magnesium responsive gene significantly overexpressed in placentas of preeclamptic women.
作者信息
Kolisek Martin, Galaviz-Hernández Carlos, Vázquez-Alaniz Fernando, Sponder Gerhard, Javaid Saba, Kurth Katharina, Nestler Axel, Rodríguez-Moran Martha, Verlohren Stefan, Guerrero-Romero Fernando, Aschenbach Jörg Rudolf, Vormann Jürgen
机构信息
Department Molecular Physiology, Institute of Veterinary-Physiology, Free University of Berlin , Germany .
出版信息
Hypertens Pregnancy. 2013 Nov;32(4):378-89. doi: 10.3109/10641955.2013.810237. Epub 2013 Jul 11.
OBJECTIVE
To examine expression profile of magnesium responsive genes (MRGs) in placentas of normoevolutive and preeclamptic women.
METHODS
The expression profiles of MRGs were determined in placentas of normoevolutive (N=26) and preeclamptic (N=25) women by RT-qPCR.
RESULTS
Among all tested MRGs (9) only SLC41A1 (encoding for Na(+)/Mg(2+) exchanger) was significantly overexpressed in ~54.2% of preeclamptic (n=24) and in ~9.5% of normoevolutive (n=21) specimens. On average, SLC41A1 was overexpressed sixfold in the preeclamptic group. Presence of SLC41A1 in placentas was confirmed by Western blot analysis. CONCLUSION. SLC41A1 is significantly overexpressed in nearly 55% of preeclamptic placentas. This may indicate a direct contribution of changed Mg homeostasis in the development of preeclampsia.
目的
研究正常妊娠和子痫前期孕妇胎盘组织中镁反应基因（MRGs）的表达谱。
方法
采用逆转录定量聚合酶链反应（RT-qPCR）检测正常妊娠（N = 26）和子痫前期（N = 25）孕妇胎盘组织中MRGs的表达谱。
结果
在所有检测的9个MRGs中，只有溶质载体家族41成员1（SLC41A1，编码Na⁺/Mg²⁺交换体）在约54.2%的子痫前期样本（n = 24）和约9.5%的正常妊娠样本（n = 21）中显著过表达。子痫前期组中，SLC41A1平均过表达6倍。蛋白质免疫印迹分析证实了胎盘组织中存在SLC41A1。结论：SLC41A1在近55%的子痫前期胎盘中显著过表达。这可能表明镁稳态变化在子痫前期发病机制中起直接作用。
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