Department of Molecular Medicine and Medical Biotechnology, Medicine School, University of Naples Federico II, via Pansini, 80131, Naples, Italy.
J Microbiol Methods. 2013 Sep;94(3):262-6. doi: 10.1016/j.mimet.2013.07.001. Epub 2013 Jul 9.
Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry (MS) fingerprinting has recently become an effective instrument for rapid microbiological diagnostics and in particular for identification of micro-organisms directly in a positive blood culture. The aim of the study was to evaluate a collection of 82 stored yeast isolates from bloodstream infection, by MALDI-TOF MS; 21 isolates were identified also directly from positive blood cultures and in the presence of other co-infecting micro-organisms. Of the 82 isolates grown on plates, 64 (76%) were correctly identified by the Vitek II system and 82 (100%) by MALDI-TOF MS; when the two methods gave different results, the isolate was identified by PCR. MALDI-TOF MS was unreliable in identifying two isolates (Candida glabrata and Candida parapsilosis) directly from blood culture; however, direct analysis from positive blood culture samples was fast and effective for the identification of yeast, which is of great importance for early and adequate treatment.
基质辅助激光解吸电离飞行时间（MALDI-TOF）质谱（MS）指纹图谱最近已成为一种快速微生物诊断的有效工具，特别是可直接对阳性血培养物中的微生物进行鉴定。本研究的目的是通过 MALDI-TOF MS 对 82 株来自血流感染的储存酵母分离株进行评估；其中 21 株也直接从阳性血培养物中鉴定出来，同时存在其他合并感染的微生物。在平板上生长的 82 株分离株中，64 株（76%）被 Vitek II 系统正确识别，82 株（100%）被 MALDI-TOF MS 识别；当两种方法给出不同的结果时，通过 PCR 对分离株进行鉴定。MALDI-TOF MS 直接从血培养物中鉴定两种分离株（光滑假丝酵母和近平滑假丝酵母）的结果不可靠；然而，直接对阳性血培养样本进行分析可快速有效地鉴定酵母，这对于早期和适当的治疗非常重要。
