Departamento de Clínica e Cirurgia Veterinárias, Escola de Veterinária, Universidade Federal de Minas Gerais, Av. Antônio Carlos 6627, Pampulha, CEP: 30123-970, Belo Horizonte, MG, Brazil.
Vet Parasitol. 2013 Nov 8;197(3-4):665-9. doi: 10.1016/j.vetpar.2013.06.014. Epub 2013 Jun 20.
This study reports the first evidence of infection by Leishmania infantum in Equus caballus in Americas and the first mixed infection of L. infantum/Leishmania braziliensis on this mammalian species in the world. The diagnoses was based on presence of parasites in lesions and bone marrow aspirates, their identification by using specific primers for L. infantum and L. braziliensis complexes and also serological methods IFAT and ELISA. The analysis of the PCR products suggested mixed infection in three animals. Further studies involving equine leishmaniasis are carrying out in order to clarify the dynamic of Leishmania sp. in this mammalian specie and their role in the transmission of those parasites in urban endemic area of Belo Horizonte, Minas Gerais State, Brazil.
本研究报告了美洲首次在马属动物中发现感染利什曼原虫和首次在该哺乳动物物种中发现利什曼原虫/利什曼原虫混合感染的证据。诊断基于病变和骨髓抽吸物中寄生虫的存在,使用针对利什曼原虫和利什曼原虫复合体的特定引物以及 IFAT 和 ELISA 血清学方法进行鉴定。PCR 产物分析表明,有三只动物存在混合感染。为了澄清巴西米纳斯吉拉斯州贝洛奥里藏特城市流行地区这些哺乳动物中利什曼原虫的动态及其在这些寄生虫传播中的作用,正在进行涉及马利什曼病的进一步研究。