Department of Environmental Engineering, Technical University of Denmark, 113 Miljoevej, 2800 Kongens Lyngby, Denmark.
Bioresour Technol. 2013 Sep;143:598-607. doi: 10.1016/j.biortech.2013.06.056. Epub 2013 Jun 22.
The newly isolated extreme thermophilic ethanologen Thermoanaerobacter pentosaceus was immobilized in different support materials in order to improve its ethanol production ability. In batch fermentation, a maximum ethanol yield of 1.36 mol mol(-1) consumed sugars was obtained by T. pentosaceus immobilized on rapeseed straw. Additionally, immobilized T. pentosaceus' ethanol production was improved by 11% in comparison to free cells. In continuous mode, it was shown that hydraulic retention time (HRT) affected ethanol yield, and a dramatic shift from ethanol to acetate and lactate production occurred at an HRT of 6 h. The maximum ethanol yield and concentration, 1.50 mol mol(-1) consumed sugars and 12.4 g l(-1), were obtained with an HRT of 12 h. The latter represented an improvement of 60% in relation to previously obtained results. This indicates that immobilization of T. pentosaceus is an effective strategy to improve its ethanol production ability.
为了提高极端嗜热乙醇发酵菌 Thermoanaerobacter pentosaceus 的乙醇生产能力，将其固定在不同的载体材料中。在分批发酵中，通过油菜秸秆固定化 Thermoanaerobacter pentosaceus 获得了 1.36 mol mol(-1)消耗糖的最大乙醇产率。此外，与游离细胞相比，固定化 Thermoanaerobacter pentosaceus 的乙醇产量提高了 11%。在连续模式下，结果表明水力停留时间（HRT）会影响乙醇产率，在 HRT 为 6 h 时，乙醇会剧烈地转化为乙酸和乳酸。当 HRT 为 12 h 时，获得了最大的乙醇产率和浓度，分别为 1.50 mol mol(-1)消耗糖和 12.4 g l(-1)。与之前的结果相比，后者提高了 60%。这表明固定化 Thermoanaerobacter pentosaceus 是提高其乙醇生产能力的有效策略。
