Chemical Engineering Department, Brigham Young University, Provo, UT, USA.
Biochemistry and Biology Departments, University of Utah, Salt Lake City, UT, USA.
Nanomedicine. 2014 Jan;10(1):67-76. doi: 10.1016/j.nano.2013.06.011. Epub 2013 Jul 9.
This study describes a novel nanocarrier of emulsion liposomes (eLiposomes) composed of a perfluoropentane nanodroplet within the aqueous interior of a DPPC liposome, along with the anticancer drug doxorubicin (Dox). The eLiposome containing Dox (eLipoDox) displayed good release of Dox upon insonation with low intensity ultrasound at 20-kHz, 1.0-MHz and 3.0-MHz. More release occurs in vitro at 20-kHz than at the higher frequencies. Controlled delivery was demonstrated by applying ultrasound (US) to HeLa tumor cells in vitro. The confocal images of Dox release to cells indicate that eLipoDox is an effective carrier of chemotherapeutic agent, and releases Dox to the cell cytosol upon insonation. This novel drug delivery system promises to provide more effective US therapy and tumor treatment and has the potential to reduce the side effects of cardiotoxicity caused by Dox.
In this paper, an ultrasound-sensitive doxorubicine-carrying nanoliposome delivery system is reported. Doxorubicin release as a result of ultrasound exposure is clearly demonstrated, paving the way to potential clinical applications with the aim of reducing the systemic toxicity and enhanced local delivery of this compound.
本研究描述了一种新型的乳状脂质体（eLiposomes）纳米载体，由全氟戊烷纳米液滴组成，位于 DPPC 脂质体的水相内部，同时还包含抗癌药物阿霉素（Dox）。载有 Dox 的 eLiposome（eLipoDox）在 20kHz、1.0MHz 和 3.0MHz 的低强度超声下显示出良好的 Dox 释放效果。在体外，20kHz 下的释放量高于较高频率。通过在体外向 HeLa 肿瘤细胞施加超声（US），实现了受控释放。Dox 释放到细胞的共聚焦图像表明，eLipoDox 是一种有效的化疗药物载体，在超声照射下将 Dox 释放到细胞质中。这种新型药物输送系统有望提供更有效的超声治疗和肿瘤治疗，并有可能降低 Dox 引起的心脏毒性的副作用。
本文报道了一种超声敏感的阿霉素载纳米脂质体递药系统。明显证实了阿霉素由于超声暴露而释放，为减少该化合物的全身毒性和增强局部递送的潜在临床应用铺平了道路。
