Eck Institute for Global Health and Department of Biological Sciences, University of Notre Dame, Notre Dame, IN 46556, USA.
J Hered. 2013 Sep-Oct;104(5):649-55. doi: 10.1093/jhered/est040. Epub 2013 Jul 11.
We report here the development of 65 novel microsatellite loci and construction of a composite genetic linkage map for Culex pipiens complex mosquitoes. Microsatellites were identified by in silico screening of the Culex quinquefasciatus genome assembly. Cross-species utility of 73 microsatellites for population studies in C. pipiens sensu stricto and C. quinquefasciatus was evaluated by genotyping a subset of samples collected in Indiana, United States, and Point Fortin, Trinidad. Allele frequencies of 67 microsatellites were within Hardy-Weinberg expectations in both population subsets. A composite linkage map was constructed based on restriction fragment length polymorphism and microsatellite polymorphisms in 12 independent F1 intercross mapping populations. The composite map consists of 61 marker loci totaling 183.9 cM distributed across the 3 linkage groups. These loci cover 29.5, 88.8, and 65.6 cM on chromosomes I-III, respectively, and allow for assignment of 10.4% of the genome assembly and 13.5% of the protein coding genes to chromosome position. Our results suggest that these microsatellites will be useful for mapping and population studies of 2 pervasive species in the C. pipiens complex. Moreover, the composite map presented here will serve as a basis for the construction of high-resolution genetic and physical maps, as well as detection of quantitative trait loci to aid in the investigation of complex genetic traits influencing phenotypes of interest.
我们在此报告了为库蚊复合体蚊子开发 65 个新的微卫星位点和构建复合遗传连锁图谱的情况。通过对库蚊基因组组装进行计算机筛选，鉴定了微卫星。通过对在美国印第安纳州和特立尼达的蓬特福德收集的部分样本进行基因分型，评估了 73 个微卫星在库蚊亚种和库蚊中的种群研究中的交叉物种通用性。在这两个种群亚群中，67 个微卫星的等位基因频率均符合哈迪-温伯格期望。基于 12 个独立的 F1 互交作图群体中的限制片段长度多态性和微卫星多态性构建了一个复合连锁图谱。该复合图谱包含 61 个标记基因座，总计 183.9cM，分布在 3 个连锁群上。这些基因座分别在染色体 I-III 上覆盖 29.5、88.8 和 65.6cM，允许将基因组组装的 10.4%和编码基因的 13.5%分配到染色体位置。我们的结果表明，这些微卫星将有助于对库蚊复合体中 2 种普遍存在的物种进行作图和种群研究。此外，本文提出的复合图谱将作为构建高分辨率遗传图谱和物理图谱以及检测数量性状基因座的基础，以帮助研究影响感兴趣表型的复杂遗传特征。
