Mori A, Severson D W, Christensen B M
Department of Animal Health and Biomedical Sciences, University of Wisconsin-Madison, USA.
J Hered. 1999 Jan-Feb;90(1):160-4. doi: 10.1093/jhered/90.1.160.
We report construction of a comparative linkage map for the mosquito (Culex pipiens) based on restriction fragment length polymorphisms (RFLPs) using cDNA clones from Aedes aegypti as probes to Southern blots of Cx. pipiens genomic DNA. Seventy-one cDNA clones were screened for hybridization and genetic diversity among three Cx. pipiens strains. Fifty-two of 71 cDNA clones, isolated from and previously mapped in Ae. aegypti (73.2%), were hybridized under high-stringency conditions with Cx. pipiens genomic DNA. Thirty-four clones (47.9%) reflected strain-specific polymorphisms. The map consists of 21 cDNA markers that identify 22 loci covering 165.8 cM. The loci mapped in Cx. pipiens cover 7.1 cM on chromosome 1, 80.4 cM on chromosome 2, and 78.3 cM on chromosome 3. Linkage relationships of the RFLP markers for chromosome 1 in Cx. pipiens are the same as chromosome 1 in Ae. aegypti, indicating that chromosome 1 is highly conserved between the two species. The comparative RFLP linkage maps for chromosomes 2 and 3 in Cx. pipiens and Ae. aegypti reflect apparent whole-arm translocations. RFLP markers for chromosome 2 in Ae. aegypti identified homologous loci on one arm of chromosomes 2 and 3 in Cx. pipiens, and RFLP markers for chromosome 3 in Ae. aegypti identified homologous loci on the opposite arms of chromosomes 2 and 3 in Cx. pipiens.
我们报道了基于限制性片段长度多态性（RFLP）构建的库蚊（致倦库蚊）比较连锁图谱，使用埃及伊蚊的cDNA克隆作为探针，对致倦库蚊基因组DNA进行Southern杂交。对71个cDNA克隆进行筛选，以检测三种致倦库蚊品系间的杂交情况和遗传多样性。71个cDNA克隆中有52个（73.2%）是从埃及伊蚊中分离并已在其基因组中定位的，这些克隆在高严谨条件下与致倦库蚊基因组DNA杂交。34个克隆（47.9%）显示出品系特异性多态性。该图谱由21个cDNA标记组成，鉴定出22个位点，覆盖165.8厘摩（cM）。致倦库蚊中定位的位点在第1号染色体上覆盖7.1 cM，在第2号染色体上覆盖80.4 cM，在第3号染色体上覆盖78.3 cM。致倦库蚊第1号染色体上RFLP标记的连锁关系与埃及伊蚊第1号染色体相同，表明这两个物种的第1号染色体高度保守。致倦库蚊和埃及伊蚊第2号和第3号染色体的比较RFLP连锁图谱显示出明显的整条染色体臂易位。埃及伊蚊第2号染色体上的RFLP标记在致倦库蚊第2号和第3号染色体的一条臂上鉴定出同源位点，埃及伊蚊第3号染色体上的RFLP标记在致倦库蚊第2号和第3号染色体的另一条臂上鉴定出同源位点。
