Yang Shuo, Li Meiqing, Lai Qiliang, Li Guizhen, Shao Zongze
Key Laboratory of Marine Genetic Resources; Fujian Key Laboratory of Marine Genetic Resources, Third Institute of Oceanography, SOA, Xiamen 361005, PR China.
School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, PR China.
Int J Syst Evol Microbiol. 2018 May;68(5):1639-1643. doi: 10.1099/ijsem.0.002612. Epub 2018 Apr 5.
A taxonomic study was carried out on strain MT13131, which was isolated from deep-sea sediment of the Indian Ocean during the screening of oil-degrading bacteria. The chain length range of n-alkanes (C8 to C32) oxidized by strain MT13131 was determined in this study. The bacterium was Gram-negative, oxidase- and catalase-positive, single rod shaped, and motile by peritrichous flagella. Growth was observed at salinities of 1-12 % and at temperatures of 10-42 °C. The isolate was capable of Tween 20, 40 and 80 hydrolysis, but incapable of gelatin, cellulose or starch hydrolysis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain MT13131 belonged to the genus Alcanivorax, with highest sequence similarity to Alcanivorax marinus R8-12 (96.92 %), other species of genus Alcanivorax shared 92.96-96.69 % sequence similarity. The principal fatty acids were summed feature 3 (C16 : 1ω6c/ω7c), summed feature 8 (C18 : 1ω7c/ω6c), C16 : 0 and C12 : 0 3OH. The G+C content of the chromosomal DNA was 64.2 mol%. Phosphatidylglycerol, phosphatidylethanolamine, three aminolipids and three phospholipids were present. The combined genotypic and phenotypic data showed that strain MT13131 represents a novel species within the genus Alcanivorax, for which the name Alcanivorax mobilis sp. nov. is proposed, with the type strain MT13131 (=MCCC 1A11581=KCTC 52985).
对菌株MT13131进行了分类学研究，该菌株是在筛选石油降解细菌过程中从印度洋深海沉积物中分离得到的。本研究确定了菌株MT13131氧化的正构烷烃（C8至C32）的链长范围。该细菌为革兰氏阴性，氧化酶和过氧化氢酶阳性，呈单杆状，具周生鞭毛，能运动。在盐度为1-12%和温度为10-42℃时观察到生长。该分离株能够水解吐温20、40和80，但不能水解明胶、纤维素或淀粉。基于16S rRNA基因序列的系统发育分析表明，菌株MT13131属于食烷菌属，与海生食烷菌R8-12的序列相似性最高（96.92%），食烷菌属的其他物种序列相似性为92.96-96.69%。主要脂肪酸为总和特征3（C16:1ω6c/ω7c）、总和特征8（C18:1ω7c/ω6c）、C16:0和C12:0 3OH。染色体DNA的G+C含量为64.2mol%。存在磷脂酰甘油、磷脂酰乙醇胺、三种氨基脂质和三种磷脂。综合基因型和表型数据表明，菌株MT13131代表食烷菌属内的一个新物种，为此提出新名称食烷游动菌（Alcanivorax mobilis sp. nov.），模式菌株为MT13131（=MCCC 1A11581=KCTC 52985）。
