Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China.
Department of Ocean Technology, College of Marine and Biology Engineering, Yancheng Institute of Technology, Yancheng, 224051, China; Key Laboratory of Applied Biology and Aquaculture of Fish in Northern China of Liaoning Province, Dalian Ocean University, Dalian, 116023, China.
Fish Shellfish Immunol. 2023 Jul;138:108826. doi: 10.1016/j.fsi.2023.108826. Epub 2023 May 16.
Herpesviral hematopoietic necrosis disease causes by cyprinid herpesvirus 2 (CyHV-2) infection is a high mortality disease that leads to great economic damage to gibel carp, Carassius auratus gibelio aquaculture. In this study, an attenuated strain of CyHV-2 G-RP7 was achieved by subculture on RyuF-2 cells derived from the fin of Ryukin-variety goldfish and GiCF cells derived from fin of gibel carp. As the attenuated vaccine candidate, there are no clinical symptoms of gibel carp that immersion or intraperitoneal injection with G-RP7 strain. The protection rates of G-PR7 to gibel carp by immersion and intraperitoneal injection were 92% and 100%, respectively. In the test for virulence reversion, the candidate was propagated through gibel carp six times by intraperitoneal injection with kidney and spleen homogenate of the inoculated fish. During in vivo passages in gibel carp, no abnormality and mortality of the inoculated fish were observed, and the virus DNA copies maintain a low level from the first passage to the sixth passage. The dynamic of virus DNA in each tissue of G-RP7 vaccination fish increased within 1, 3, and 5 days post-immunization, and subsequently decreased and stabilized within 7 and 14 days. In addition, the increase of anti-virus antibody titer was detected both immersion and injection immunization fish 21 days after vaccination by ELISA. These results demonstrated that G-RP7 can be a promising live attenuated vaccine candidate against the disease.
由鲤鱼疱疹病毒 2 (CyHV-2)感染引起的疱疹病毒性造血坏死病是一种高死亡率疾病，给鲤鱼、黄河鲤鱼的养殖造成了巨大的经济损失。在本研究中，通过在鲤鱼品系锦鲤的鳍衍生的 RyuF-2 细胞和鲤鱼的鳍衍生的 GiCF 细胞上连续传代，获得了 CyHV-2 的减毒株 G-RP7。作为减毒疫苗候选株，经浸泡或腹腔注射 G-RP7 菌株的鲤鱼没有临床症状。浸泡和腹腔注射 G-PR7 对鲤鱼的保护率分别为 92%和 100%。在毒力返祖试验中，候选株通过腹腔注射接种鱼的肾和脾匀浆在鲤鱼体内传代 6 次。在鲤鱼体内传代过程中，接种鱼没有出现异常和死亡，病毒 DNA 拷贝从第 1 代到第 6 代保持低水平。G-RP7 疫苗接种鱼各组织中病毒 DNA 的动态在免疫后 1、3 和 5 天内增加，随后在 7 和 14 天内减少并稳定。此外，通过 ELISA 在免疫后 21 天检测到浸泡和注射免疫鱼的抗病毒抗体滴度增加。这些结果表明，G-RP7 可以成为一种有前途的针对该疾病的活减毒疫苗候选株。
