Ben-Zaken Sigal, Meckel Yoav, Nemet Dan, Eliakim Alon
The Zinman College of Physical Education and Sports Sciences at the Wingate Institute, Genetics and Molecular Biology Laboratory, Netanya 42902, Israel.
Growth Horm IGF Res. 2013 Oct;23(5):175-8. doi: 10.1016/j.ghir.2013.06.005. Epub 2013 Jul 11.
Insulin-like growth factor-I (IGF-I) plays a key role in exercise-associated muscle growth and development. The regulatory region of the promoter of the IGF-I gene is labile, but changes in this region were studied mostly in the elderly and in relation to pathological states. C-1245T (rs35767) is a genetic variation in the promoter region of the IGF-I gene. The minor allele T was found to be associated with higher circulating IGF-I levels, and possibly with increased muscle mass. The aim of the current study was to analyze the frequency distribution of C-1245T SNP in athletic and nonathletic Israeli populations.
One hundred and sixty-five athletes (78 endurance-type athletes, and 87 power-type athletes) and 159 nonathletic healthy individuals participated in the current study. Genomic DNA was extracted from peripheral EDTA treated anti-coagulated blood using a standard protocol. Genotyping of the IGF1 C-1245T polymorphism was performed using polymerase chain reaction (PCR).
We found that the endurance and power athletes' allele and genotype frequencies were significantly different from those of the control group. Only 4.8% of the athletes were TT carriers, but none of the controls carried this genotype. The T allele was found to be more frequent in the top-level power athletes (international and Olympic level) compared to national level athletes, but such a difference was not found in endurance athletes.
Our findings suggest a possible contribution for the relatively rare IGF-I TT genotype to endurance performance, and in particular to power sport excellence in Israeli athletes.
胰岛素样生长因子-I（IGF-I）在运动相关的肌肉生长和发育中起关键作用。IGF-I基因启动子的调控区域不稳定，但该区域的变化大多在老年人中以及与病理状态相关的研究中进行。C-1245T（rs35767）是IGF-I基因启动子区域的一种基因变异。发现次要等位基因T与较高的循环IGF-I水平相关，并且可能与肌肉量增加有关。本研究的目的是分析以色列运动员和非运动员群体中C-1245T单核苷酸多态性（SNP）的频率分布。
165名运动员（78名耐力型运动员和87名力量型运动员）和159名非运动员健康个体参与了本研究。使用标准方案从经EDTA处理的外周抗凝血中提取基因组DNA。使用聚合酶链反应（PCR）对IGF1 C-1245T多态性进行基因分型。
我们发现耐力型和力量型运动员的等位基因和基因型频率与对照组有显著差异。只有4.8%的运动员是TT携带者，但对照组中没有人携带这种基因型。与国家级运动员相比，顶级力量型运动员（国际级和奥运级）中T等位基因更为常见，但耐力型运动员中未发现这种差异。
我们的研究结果表明，相对罕见的IGF-I TT基因型可能对以色列运动员的耐力表现，特别是力量运动的卓越表现有贡献。
