Okabe Yuka, Lee Abraham P
1Department of Biomedical Engineering, University of California-Irvine, Irvine, CA, USA.
J Lab Autom. 2014 Apr;19(2):163-70. doi: 10.1177/2211068213495546. Epub 2013 Jul 12.
We present a novel method to fragment DNA by using lateral cavity acoustic transducers (LCATs). DNA solution is placed within a microfluidic device containing LCATs. The LCATs cause microstreaming, which fragments DNA within the solution without any need for purification or downstream processing. The LCAT-based DNA fragmentation method offers an easy-to-use, low-cost, low-energy way to fragment DNA that is amenable to integration on microfluidic platforms to further automate DNA processing. Furthermore, the LCAT microdevice requires less than 10 µL of sample, and no external equipment is needed besides a piezoelectric transducer.
我们提出了一种使用横向腔声换能器（LCAT）对DNA进行片段化的新方法。将DNA溶液置于含有LCAT的微流控装置中。LCAT会引起微流，从而在无需任何纯化或下游处理的情况下使溶液中的DNA片段化。基于LCAT的DNA片段化方法提供了一种易于使用、低成本、低能耗的DNA片段化方式，适合集成到微流控平台上以进一步实现DNA处理的自动化。此外，LCAT微型装置所需样品量不到10微升，除了压电换能器外无需其他外部设备。
