School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Republic of Korea.
J Photochem Photobiol B. 2013 Aug 5;125:194-201. doi: 10.1016/j.jphotobiol.2013.06.009. Epub 2013 Jun 27.
With the aim to establish a novel nanocarrier system with relatively high payload and high photoprotection capacity for photo-labile active compounds, in this work, deltamethrin (photo-labile compound) was encapsulated into corn oil-nanoemulsions (NE) by a hot high pressure homogenization technique followed by coating with chitosan as the first coating layer (CH-NE) and lignosulfonate as the second coating layer to form a double-layer coated NE (L-CH-NE). The optimal conditions for preparation of NE, chitosan coating and lignosulfonate coating were investigated. The results indicate that polymer coating and the number of coating layers significantly affected the release profile and photoprotection capacity of nanocarriers. In particular, after coating, the release rate became slower and photoprotection capacity became higher. Moreover, in the case of L-CH-NE after 24h of UV exposure in direct photolysis and 2.5h of UV exposure in indirect photolysis, the non-degraded amount of deltamethrin was approximately 4.5 times and 2.1 times, respectively, higher than that of the free-from deltamethrin. In the future, this novel nanocarrier system will show great potential and be widely applied to many fields related to protection of photo-labile compounds against photo-degradation.
为了建立一种新型的纳米载体系统，该系统具有相对较高的载药量和高光保护能力，适用于光不稳定的活性化合物，本工作采用高压均质热法将拟除虫菊酯（光不稳定化合物）包封到玉米油纳米乳液（NE）中，然后用壳聚糖作为第一层涂层（CH-NE）和木质素磺酸钠作为第二层涂层形成双层包覆的 NE（L-CH-NE）。研究了制备 NE、壳聚糖涂层和木质素磺酸钠涂层的最佳条件。结果表明，聚合物涂层和涂层层数显著影响纳米载体的释放曲线和光保护能力。特别是在涂层之后，释放速率变得更慢，光保护能力变得更高。此外，在直接光解 24h 和间接光解 2.5h 后，L-CH-NE 中除虫菊酯的未降解量分别比游离除虫菊酯高约 4.5 倍和 2.1 倍。在未来，这种新型的纳米载体系统将具有很大的潜力，并广泛应用于许多与保护光不稳定化合物免受光降解相关的领域。
