Faculty of Health and Life Sciences, Northumbria University, Newcastle upon Tyne, UK.
Antonie Van Leeuwenhoek. 2013 Sep;104(3):435-40. doi: 10.1007/s10482-013-9958-5. Epub 2013 Jul 13.
The taxonomic position of Rhodococcus strain RHA1, an effective degrader of polychlorinated biphenyls with a large linear chromosome, was established using a polyphasic approach. The morphological and chemotaxonomic properties of the strain were typical of members of the genus Rhodococcus. The strain shared a high level of 16S rRNA sequence similarity (99.9 %) with the type strain of Rhodococcus jostii, a member of the Rhodococcus erythropolis subclade. The two strains shared a DNA:DNA relatedness value well above the cut-off point recommended for the circumscription of genomic species and had a broad range of phenotypic properties in common. The combination of genomic and phenotypic data show strain RHA1 to be a bona fide member of the species Rhodococcus jostii.
利用多相分类法确定了具有大线性染色体的有效降解多氯联苯的红球菌 RHA1 菌株的分类地位。该菌株的形态和化学分类特征均为红球菌属的典型特征。该菌株与红球菌属的红球菌 jostii 型菌株的 16S rRNA 序列相似性很高(99.9%),后者属于红杆菌亚群。这两个菌株的 DNA:DNA 同源性值远远高于基因组物种界定的建议临界点,并且具有广泛的共同表型特征。基因组和表型数据的组合表明 RHA1 菌株是真正的红球菌 jostii 种的成员。