Mayilraj S, Krishnamurthi S, Saha P, Saini H S
Microbial Type Culture Collection and Gene Bank (MTCC), Institute of Microbial Technology, Chandigarh 160 036, India.
Department of Microbiology, Guru Nanak Dev University (GNDU), Amritsar 143 005, India.
Int J Syst Evol Microbiol. 2006 May;56(Pt 5):979-982. doi: 10.1099/ijs.0.63831-0.
The taxonomic position of an actinomycete, strain K07-23T, isolated from a cold desert of the Himalayas, India, was established by a polyphasic approach. The strain exhibited phenotypic characters that were typical of the genus Rhodococcus. 16S rRNA gene sequence (1467 bases) comparisons confirmed that strain K07-23T belongs to the genus Rhodococcus. 16S rRNA sequence similarity studies showed that the isolate is very closely related to Nocardia corynebacterioides DSM 20151T (98.6 %), which has been recently reclassified as Rhodococcus corynebacterioides. It showed 94.4-96.6 % sequence similarity with other species of the genus Rhodococcus. However, genomic relatedness between strain K07-23T and R. corynebacterioides as revealed by DNA-DNA hybridization was low (62 %). Based on polyphasic analysis, strain K07-23T could be clearly distinguished from other species. It is proposed that strain K07-23T (=MTCC 6634T=DSM 44908T=JCM 13011T) represents a novel species of Rhodococcus, Rhodococcus kroppenstedtii sp. nov.
通过多相分类方法确定了从印度喜马拉雅山寒冷沙漠中分离出的一株放线菌K07-23T的分类地位。该菌株表现出红球菌属典型的表型特征。16S rRNA基因序列（1467个碱基）比较证实菌株K07-23T属于红球菌属。16S rRNA序列相似性研究表明，该分离株与棒状诺卡氏菌DSM 20151T密切相关（98.6%），后者最近已重新分类为棒状红球菌。它与红球菌属的其他物种的序列相似性为94.4 - 96.6%。然而，DNA - DNA杂交显示菌株K07-23T与棒状红球菌之间的基因组相关性较低（62%）。基于多相分析，菌株K07-23T可与其他物种明确区分。建议将菌株K07-23T（=MTCC 6634T=DSM 44908T=JCM 13011T）代表红球菌属的一个新物种，命名为克氏红球菌（Rhodococcus kroppenstedtii sp. nov.） 。
