Department of Surgery, Eastern Hepatobiliary Hospital, Shanghai, China.
Pathol Oncol Res. 2013 Oct;19(4):815-20. doi: 10.1007/s12253-013-9649-6. Epub 2013 Jul 14.
Metastasis suppressor 1 (MTSS1) is a novel metastasis suppressor gene in a variety of cancers. This study aimed to detect MTSS1 expression in normal and cancerous tissue specimens from Chinese patients with hilarcholangiocarcinoma to determine the association with clinicopathological parameters and survival. Tissue microarrays containing normal and tumor specimens were constructed using paraffin blocks from 61 patients for immunohistochemical analysis of MTSS1 expression. A subgroup of these tissues was verified by Western blot analysis. MTSS1 protein was expressed in 24 of 61 cases (39.3 %) of tumor tissues, compared to that in 22 of 26 (84.6 %) of non-neoplastic bile duct epithelium and in 26 of 26 (100 %) of adjacent normal liver cells. Loss of MTSS1 expression was associated with lymph node metastases of cholangiocelluarcarcinoma and tumorcellde-differentiation.MTSS1 expression inversely associated with tumor recurrence and overall survival of the patients by univariate and multivariate analyses. MTSS1 expression was significantly decreased in human hilarcholangiocarcinoma and lost MTSS1 expression was associated with pooroverall survival and tumor recurrences in cholangiocarcinoma patients. Thus, MTSS1 expression represents an independent predictor for tumor recurrence and overall survival in patients with cholangiocarcinoma.
转移抑制因子 1（MTSS1）是多种癌症中一种新的转移抑制基因。本研究旨在检测中国肝内胆管癌患者正常和癌组织标本中 MTSS1 的表达，以确定其与临床病理参数和生存的关系。使用石蜡块构建包含正常和肿瘤标本的组织微阵列，用于 MTSS1 表达的免疫组织化学分析。对这些组织的一个亚组进行了 Western blot 分析。在 61 例肿瘤组织中的 24 例（39.3%）、26 例非肿瘤胆管上皮组织中的 22 例（84.6%）和 26 例相邻正常肝细胞中检测到 MTSS1 蛋白表达。MTSS1 表达缺失与胆管细胞癌的淋巴结转移和肿瘤细胞分化有关。单因素和多因素分析表明，MTSS1 表达与患者的肿瘤复发和总生存呈负相关。MTSS1 在人肝内胆管癌中表达明显降低，MTSS1 表达缺失与胆管癌患者的总生存和肿瘤复发不良相关。因此，MTSS1 表达是胆管癌患者肿瘤复发和总生存的独立预测因子。
