Virology Unit, Institute of Inflammation and Repair, The University of Manchester, Manchester, United Kingdom.
J Med Virol. 2013 Nov;85(11):1947-54. doi: 10.1002/jmv.23678. Epub 2013 Jul 12.
In this study, real-time PCR was used to quantify adenovirus DNA in cell suspensions prepared from 106 right and left tonsils and 10 adenoids obtained from 57 patients who underwent routine tonsillectomies and/or adenoidectomies. Eighty-four (72.4%) tonsils and adenoids samples were positive for HAdV by real-time PCR. The viral load ranged from 2.8 × 10(2) to 2.6 × 10(6) copies/10(7) cells and varied up to sixfold between the right and left tonsils. In some cases, only one tonsil was positive and the viral load was lower in older children. Seventy-eight of 84 positive samples could be typed by sequencing of the hexon L1 region. Species C (types 1, 2, and 5) were detected in 84.1% of the patients followed by types 3 and 7 of species B (6.8%), HAdV-E4 (6.8%), and HAdV-F41 (2.3%). In one patient adenovirus C2 was found in the left tonsil and adenovirus C5 in the right tonsil. No DNA methylation was detected in either the E1A promoter or the major late promoter region of adenovirus DNA from six tonsils and adenoids samples and two clinical isolates.
在这项研究中，我们使用实时 PCR 定量分析了 57 例行扁桃体切除术和/或腺样体切除术患者的 106 个右扁桃体和左扁桃体以及 10 个腺样体细胞悬液中的腺病毒 DNA。84（72.4%）个扁桃体和腺样体样本通过实时 PCR 呈 HAdV 阳性。病毒载量范围为 2.8×10(2) 至 2.6×10(6) 拷贝/10(7) 个细胞，左右扁桃体之间的差异可达 6 倍。在某些情况下，只有一个扁桃体呈阳性，且年长儿童的病毒载量较低。84 个阳性样本中的 78 个可以通过六邻体 L1 区的测序进行分型。C 种（1、2 和 5 型）在 84.1%的患者中被检测到，其次是 B 种的 3 和 7 型（6.8%）、HAdV-E4（6.8%）和 HAdV-F41（2.3%）。在一名患者中，左扁桃体中发现了腺病毒 C2，右扁桃体中发现了腺病毒 C5。从 6 个扁桃体和腺样体样本以及 2 个临床分离株的腺病毒 DNA 的 E1A 启动子或主要晚期启动子区域均未检测到 DNA 甲基化。
