Zhao Jing, Fan Yu-chen, Zhang Feng, Yang Yang, Zhao Ze-hua, Sun Feng-kai, Wang Kai
Department of Hepatology, Qilu Hospital of Shangdong University, Jiang, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Feb;27(1):47-9.
This study is aimed to investigate oxidative stress status in chronic hepatitis C (CHC) patients.
52 CHC patients were divided into two groups according to the serum level of alanine aminotransferase (ALT): group A (elevated ALT group) and group B (normal ALT group). 20 healthy controls were included in this study. Serum levels of xanthine oxidase (XOD), malondialdehyde (MDA), oxidizided glutathione (GSSG), glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione S-transferase (GST), glutathione reductase (GR) and vitamin C (Vc) were determined by enzyme-linked immunosorbent assay (ELISA).
Serum levels of XOD, MDA, GST and GR increased in CHC patients compared with healthy controls. While, serum levels of GSH, GSH-Px and Vc decreased compared with healthy controls. Furthermore, serum levels of XOD, MDA, GSSG, GST and GR in group A were up-regulated compared with group B. Serum levels of GSH, GSH-Px and Vc in group A were down-regulated compared with group B. In CHC patients, serum ALT level positively correlated with serum levels of XOD, MDA, GSSG and GST, while, negatively correlated with serum levels of GSH, GSH-Px and Vc. Serum aspartate aminotransferase (AST) level positively correlated with serum levels of XOD, MDA, GSSG, GR and GST, while, negatively correlated with serum GSH-Px level in CHC patients. Serum gamma-glutamyl transpeptidase (GGT) level positively correlated with serum GR level and negatively correlated with serum GSH level in CHC patients. Serum alkaline phosphatase (AKP) level positively correlated with serum levels of MDA and GR in CHC patients. In CHC patients, serum XOD level was positively related with serum HCV RNA level.
Oxidative stress was increased in CHC patients. In CHC patients with elevated serum ALT level, oxidative stress usually became serious.
本研究旨在调查慢性丙型肝炎（CHC）患者的氧化应激状态。
52例CHC患者根据血清丙氨酸氨基转移酶（ALT）水平分为两组：A组（ALT升高组）和B组（ALT正常组）。本研究纳入20名健康对照者。采用酶联免疫吸附测定（ELISA）法测定血清黄嘌呤氧化酶（XOD）、丙二醛（MDA）、氧化型谷胱甘肽（GSSG）、谷胱甘肽（GSH）、谷胱甘肽过氧化物酶（GSH-Px）、谷胱甘肽S-转移酶（GST）、谷胱甘肽还原酶（GR）和维生素C（Vc）水平。
与健康对照者相比，CHC患者血清XOD、MDA、GST和GR水平升高。而与健康对照者相比血清GSH、GSH-Px和Vc水平降低。此外，A组血清XOD、MDA、GSSG、GST和GR水平相对于B组上调。A组血清GSH,G SH-Px和Vc水平相对于B组下调。在CHC患者血清 ALT水平与血清X OD、MDA,G SS G and GST水平成正相关，而与血清G SH、G SH-P ox and Vc水平成负相关。在CHC患者血清天门冬氨酸氨基转移酶(A ST))水平与血清X OD、MDA、G SS G GR and GST成正相关，而与血清谷胱甘肽过氧化物酶水平成负相关。血清γ-谷氨酰转肽酶(GGT)水平与CHC患者血清GR水平成正相关，与血清谷胱甘肽水平成负负相关性。血清碱性磷酸酶( AKP)水平与CHC患者血清MDA和GR水平呈正相关。在CHC患者血清XOD水平与血清HCV RNA水平呈正相关性。
CHC患者氧化应激增加。在血清 ALT水平升高的CHC患者氧化应激通常更为严重。
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