Liu Jun, Zhang Guo-Xiang
Xiaoshan Hospital of Zhejiang, Hangzhou 311201, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2013 Feb;27(1):67-9.
To establish a hepatitis C virus (HCV) diagnostic assay using a protein array based on the quantum dots (QDs) encoded microbeads.
Using QDs encoded microbeads array and immunofluorescence techniques, the highly purified HCV NS3, NS4, NS5 and Core protein were respectively immobilized on the surface of encoded beads, which were used for the detection of anti-HCV antibody in serum. To evaluate the microbeads protein array, 120 HCV positive and 50 HCV negative samples were tested, and compared with recombinant immunoblot assay(RIBA) results as golden standard. The sensitivity, specificity and accuracy value were calculated.
Compared 120 positive samples detected with RIBA, the sensitivity of microbeads array is 97.50% (117/120), the specificity is 96.0% (48/50), and accuracy is 97. 06% [(117 + 48)/(120 + 50)], The sensitivity of microbeads protein array is similar with RIBA methods. In the 120 positive samples tested with protein array, the positive rate of anti-HCV Core is 92. 50% (111/120) , the positive rate of anti-HCV NS3 is 89. 17% (107/120), the positive rate of anti-HCV NS4 is 70. 83% (85/120), the positive rate of anti-HCV NS5 is 52.50% (63/120).
建立一种基于量子点（QD）编码微珠的蛋白质芯片丙型肝炎病毒（HCV）诊断检测方法。
采用QD编码微珠芯片和免疫荧光技术，将高度纯化的HCV NS3、NS4、NS5和核心蛋白分别固定在编码微珠表面，用于检测血清中的抗HCV抗体。为评估微珠蛋白质芯片，对120份HCV阳性和50份HCV阴性样本进行检测，并与作为金标准的重组免疫印迹法（RIBA）结果进行比较。计算灵敏度、特异性和准确度值。
与RIBA检测的120份阳性样本相比，微珠芯片的灵敏度为97.50%（117/120），特异性为96.0%（48/50），准确度为97.06%[（117 + 48）/（120 + 50）]，微珠蛋白质芯片的灵敏度与RIBA方法相似。在蛋白质芯片检测的120份阳性样本中，抗HCV核心抗体阳性率为92.50%（111/120），抗HCV NS3抗体阳性率为89.17%（107/120），抗HCV NS4抗体阳性率为70.83%（85/120），抗HCV NS5抗体阳性率为52.50%（63/120）。
