INTEC (UNL-CONICET), Güemes 3450, 3000 Santa Fe, Argentina.
INTEC (UNL-CONICET), Güemes 3450, 3000 Santa Fe, Argentina; Facultad de Ingeniería Química (UNL), Santiago del Estero 2829, 3000 Santa Fe, Argentina.
Colloids Surf B Biointerfaces. 2013 Nov 1;111:354-9. doi: 10.1016/j.colsurfb.2013.06.024. Epub 2013 Jun 26.
This paper presents an optical method for real-time monitoring of protein adsorption using porous silicon self-supported microcavities as a label-free detection platform. The study combines an experimental approach with a physical model for the adsorption process. The proposed model agrees well with experimental observations, and provides information about the kinetics of diffusion and adsorption of proteins within the pores, which will be useful for future experimental designs.
本文提出了一种使用多孔硅自支撑微腔作为无标记检测平台实时监测蛋白质吸附的光学方法。该研究结合了实验方法和吸附过程的物理模型。所提出的模型与实验观察吻合较好，提供了关于蛋白质在孔内扩散和吸附动力学的信息，这将对未来的实验设计有用。
