Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.
Biosens Bioelectron. 2013 Dec 15;50:194-201. doi: 10.1016/j.bios.2013.06.020. Epub 2013 Jun 26.
Skeletal muscle tissues play a significant role to maintain the glucose level of whole body and any dysfunction of this tissue leads to the diabetes disease. A culture medium was created in which the muscle cells could survive for a long time and meanwhile it did not interfere with the glucose sensing. We fabricated a model of skeletal muscle tissues in vitro to monitor its glucose uptake. A nanoporous gold as a high sensitive nanobiosensor was then successfully developed and employed to detect the glucose uptake of the tissue models in this medium upon applying the electrical stimulation in a rapid, and non-invasive approach. The response of the glucose sensor was linear in a wide concentration range of 1-50 mM, with a detection limit of 3 μM at a signal-to-noise ratio of 3.0. The skeletal muscle tissue was electrically stimulated during 24 h and glucose uptake was monitored during this period. During the first 3 h of stimulation, electrically stimulated muscle tissue consumed almost twice the amount of glucose than counterpart non-stimulated sample. In total, the glucose consumption of muscle tissues was higher for the electrically stimulated tissues compared to those without applying the electrical field.
骨骼肌组织在维持全身葡萄糖水平方面起着重要作用，其任何功能障碍都会导致糖尿病。我们创造了一种培养基，使肌肉细胞能够在其中长时间存活，同时不会干扰葡萄糖的感应。我们在体外构建了骨骼肌组织模型，以监测其葡萄糖摄取情况。然后，我们成功地开发了一种纳米多孔金作为高灵敏度纳米生物传感器，并采用这种方法在快速、非侵入性的方式下施加电刺激，来检测组织模型在这种培养基中的葡萄糖摄取情况。该葡萄糖传感器在 1-50mM 的宽浓度范围内呈线性响应，检测限为 3μM，信噪比为 3.0。在 24 小时内对骨骼肌组织进行电刺激，并在此期间监测葡萄糖摄取情况。在刺激的前 3 小时内，电刺激的肌肉组织消耗的葡萄糖量几乎是对照未受刺激样本的两倍。总的来说，与未施加电场的组织相比，电刺激的组织消耗的葡萄糖量更高。
