Pharmacology Division, University Institute of Pharmaceutical Sciences, UGC Centre of Advanced Study, Panjab University, Chandigarh, 160014, India.
Arch Pharm Res. 2017 Oct;40(10):1166-1175. doi: 10.1007/s12272-013-0205-4. Epub 2013 Jul 16.
Stress occurs in everyday life and persistence of it causes memory loss. Bioflavonoids like quercetin are reported to have poor bioavailability and limited therapeutic potential against stress induced neurological disorders. Therefore, the present study is an attempt to elucidate the therapeutic potency of combination of quercetin with piperine; a bioavailability enhancer against chronic unpredictable stress (CUS)-induced behavioral and biochemical alterations. Laca mice were subjected to a series of stressful events for a period of 28 days. Quercetin (20, 40 and 80 mg/kg, p.o.), piperine (20 mg/kg, p.o.) and their combinations were administered daily 30 min before CUS procedure. Piracetam (100 mg/kg, i.p.) served as a standard control. CUS caused impaired spatial navigation in Morris water maze test and poor retention in elevated plus maze task. Further, there was significant increase in brain oxidative stress markers and neuro-inflammation (TNF-α). This was coupled with marked rise in acetylcholinesterase and serum corticosterone levels. Co-administration of piperine with quercetin significantly elevated their potential to restore these behavioral, biochemical and molecular changes associated with mouse model of CUS. These results suggest that piperine enhances the neuroprotective effects of quercetin against CUS-induced oxidative stress, neuro-inflammation and memory deficits.
日常生活中会发生压力，持续的压力会导致记忆力下降。研究表明，生物类黄酮如槲皮素的生物利用度较差，对压力引起的神经紊乱的治疗潜力有限。因此，本研究试图阐明槲皮素与胡椒碱联合使用的治疗潜力；胡椒碱是一种生物利用度增强剂，可对抗慢性不可预测应激（CUS）引起的行为和生化改变。Laca 小鼠接受了一系列应激事件，持续 28 天。每天在 CUS 程序前 30 分钟给予槲皮素（20、40 和 80mg/kg，po）、胡椒碱（20mg/kg，po）及其组合。吡拉西坦（100mg/kg，ip）作为标准对照。CUS 导致 Morris 水迷宫测试中空间导航受损，高架十字迷宫任务中的保留能力差。此外，大脑氧化应激标志物和神经炎症（TNF-α）显著增加。这伴随着乙酰胆碱酯酶和血清皮质酮水平的明显升高。胡椒碱与槲皮素联合给药可显著提高其恢复与 CUS 诱导的氧化应激、神经炎症和记忆缺陷相关的行为、生化和分子变化的潜力。这些结果表明，胡椒碱增强了槲皮素对 CUS 诱导的氧化应激、神经炎症和记忆缺陷的神经保护作用。
